Literature DB >> 23201396

Comparison of two selective media for the recovery of Clostridium difficile from environmental surfaces.

K A Hill1, J Collins, L Wilson, J D Perry, F K Gould.   

Abstract

Two culture media were compared for their ability to isolate Clostridium difficile from environmental sites within a UK hospital. The media were cefoxitin-cycloserine-egg yolk agar plus lysozyme (CCEY/L) and chromID C. difficile. A wide range of environmental surfaces was sampled using sterile sponges (Polywipes) and these were inoculated on to both media. C. difficile was recovered from 105 of 496 sites (21%) using a combination of both media. The sensitivity of chromID C. difficile was 87.6% compared with 26.6% for CCEY/L (P < 0.0001). chromID C. difficile performed significantly better than CCEY/L for the recovery of C. difficile from the environment.
Copyright © 2012 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

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Year:  2012        PMID: 23201396     DOI: 10.1016/j.jhin.2012.10.006

Source DB:  PubMed          Journal:  J Hosp Infect        ISSN: 0195-6701            Impact factor:   3.926


  6 in total

1.  A Novel Quantitative Sampling Technique for Detection and Monitoring of Clostridium difficile Contamination in the Clinical Environment.

Authors:  Shanom Ali; Monika Muzslay; Peter Wilson
Journal:  J Clin Microbiol       Date:  2015-06-03       Impact factor: 5.948

Review 2.  A Decade of Development of Chromogenic Culture Media for Clinical Microbiology in an Era of Molecular Diagnostics.

Authors:  John D Perry
Journal:  Clin Microbiol Rev       Date:  2017-04       Impact factor: 26.132

Review 3.  Diagnosis of Clostridium difficile infection: an ongoing conundrum for clinicians and for clinical laboratories.

Authors:  Carey-Ann D Burnham; Karen C Carroll
Journal:  Clin Microbiol Rev       Date:  2013-07       Impact factor: 26.132

4.  Diagnosis of Clostridium difficile: real-time PCR detection of toxin genes in faecal samples is more sensitive compared to toxigenic culture.

Authors:  M B F Jensen; K E P Olsen; X C Nielsen; A M Hoegh; R B Dessau; T Atlung; J Engberg
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-11-25       Impact factor: 3.267

5.  Evaluation of a chromogenic culture medium for the detection of Clostridium difficile.

Authors:  John Jeongseok Yang; You Sun Nam; Min Jin Kim; Sun Young Cho; Eunkyung You; Yun Soo Soh; Hee Joo Lee
Journal:  Yonsei Med J       Date:  2014-07       Impact factor: 2.759

6.  Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens.

Authors:  Bo-Moon Shin; Eun Joo Lee
Journal:  Ann Lab Med       Date:  2013-12-06       Impact factor: 3.464

  6 in total

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