Literature DB >> 23200314

A soluble form of Epstein-Barr virus gH/gL inhibits EBV-induced membrane fusion and does not function in fusion.

Cynthia L Rowe1, Sarah A Connolly, Jia Chen, Theodore S Jardetzky, Richard Longnecker.   

Abstract

We investigated whether soluble EBV gH/gL (sgH/gL) functions in fusion and made a series of truncations of gH/gL domains based on the gH/gL crystal structure. We found sgH/gL failed to mediate cell-cell fusion both when co-expressed with the other entry glycoproteins and when added exogenously to fusion assays. Interestingly, sgH/gL inhibited cell-cell fusion in a dose dependent manner when co-expressed. sgH/gL from HSV was unable to inhibit EBV fusion, suggesting the inhibition was specific to EBV gH/gL. sgH/gL stably binds gp42, but not gB nor gH/gL. The domain mutants, DI/gL, DI-II/gL and DI-II-III/gL were unable to bind gp42. Instead, DI-II/gL, DI-II-III/gL and sgH/gL but not DI/gL decreased the expression of gp42, resulting in decreased overall fusion. Overall, our results suggest that domain IV may be required for proper folding and the transmembrane domain and cytoplasmic tail of EBV gH/gL are required for the most efficient fusion.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID： 23200314      PMCID： PMC3545092          DOI： 10.1016/j.virol.2012.10.039

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  40 in total

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3.  Herpes simplex virus glycoprotein D bound to the human receptor HveA.

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Review 4.  Role of Epstein-Barr virus in Burkitt's lymphoma.

Authors:  K Takada
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5.  Human cytomegalovirus glycoprotein gO complexes with gH/gL, promoting interference with viral entry into human fibroblasts but not entry into epithelial cells.

Authors:  Adam L Vanarsdall; Marie C Chase; David C Johnson
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Authors:  K M Haan; S K Lee; R Longnecker
Journal:  Virology       Date:  2001-11-10       Impact factor: 3.616

7.  Cell fusion induced by herpes simplex virus glycoproteins gB, gD, and gH-gL requires a gD receptor but not necessarily heparan sulfate.

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8.  Herpes simplex virus glycoproteins gH/gL and gB bind Toll-like receptor 2, and soluble gH/gL is sufficient to activate NF-κB.

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Authors:  Paolo Di Giovine; Ethan C Settembre; Arjun K Bhargava; Micah A Luftig; Huan Lou; Gary H Cohen; Roselyn J Eisenberg; Claude Krummenacher; Andrea Carfi
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Authors:  Jia Chen; Cynthia L Rowe; Theodore S Jardetzky; Richard Longnecker
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  11 in total

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Journal:  J Virol       Date:  2014-08-20       Impact factor: 5.103

Review 2.  The structural basis of herpesvirus entry.

Authors:  Sarah A Connolly; Theodore S Jardetzky; Richard Longnecker
Journal:  Nat Rev Microbiol       Date:  2020-10-21       Impact factor: 60.633

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Authors:  Cynthia L Rowe; Jia Chen; Theodore S Jardetzky; Richard Longnecker
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6.  Assembly and architecture of the EBV B cell entry triggering complex.

Authors:  Karthik Sathiyamoorthy; Jiansen Jiang; Yao Xiong Hu; Cynthia L Rowe; Britta S Möhl; Jia Chen; Wei Jiang; Elizabeth D Mellins; Richard Longnecker; Z Hong Zhou; Theodore S Jardetzky
Journal:  PLoS Pathog       Date:  2014-08-21       Impact factor: 6.823

7.  The Cytoplasmic Tail Domain of Epstein-Barr Virus gH Regulates Membrane Fusion Activity through Altering gH Binding to gp42 and Epithelial Cell Attachment.

Authors:  Jia Chen; Theodore S Jardetzky; Richard Longnecker
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Review 8.  Human MHC-II with Shared Epitope Motifs Are Optimal Epstein-Barr Virus Glycoprotein 42 Ligands-Relation to Rheumatoid Arthritis.

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9.  Regulation of herpes simplex virus gB-induced cell-cell fusion by mutant forms of gH/gL in the absence of gD and cellular receptors.

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10.  In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures.

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