Generation of retinal cells from pluripotent stem cells.

Retinal degeneration is a leading cause of incurable low vision and blindness worldwide. Most retinal degenerative diseases are caused by irreversible apoptosis of retinal neural cells or adjacent supporting tissue. Because there is no radical treatment for retinal degeneration, most therapies are aimed at specific situations, such as drug or surgical intervention for late complications. Retinal cell replacement would be valuable for regenerating functional retinas, and therefore it is being examined as a next-generation treatment for retinal degeneration. With advances in stem cell biology, considerable progress has been made in recent years on generation of retinal cells. Both sensory retinal neural cells and retinal pigment epithelial cells can be induced in vitro from pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells. Here, we review the stepwise differentiation of retinal cells from pluripotent stem cells, with emphases on the methodology and application potential.