Literature DB >> 23174277

MLVA-16 loci panel on Brucella spp. using multiplex PCR and multicolor capillary electrophoresis.

Giuliano Garofolo1, Massimo Ancora, Elisabetta Di Giannatale.   

Abstract

The Multi Locus Variable Number Tandem Repeat Analysis 16 loci panel (MLVA-16), involving singleplex PCRs and agarose gel electrophoresis, is the standard genotyping method for Brucella spp., used also for the Brucella international online database. We describe an alternative, reliable, high-throughput MLVA-16 protocol using multiplex PCRs and multicolor capillary electrophoresis.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 23174277     DOI： 10.1016/j.mimet.2012.11.007

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  18 in total

1.  Human brucellosis in Maghreb: existence of a lineage related to socio-historical connections with Europe.

Authors:  Nedjma Lounes; Moulay-Ali Cherfa; Gilles Le Carrou; Abdellah Bouyoucef; Maryne Jay; Bruno Garin-Bastuji; Virginie Mick
Journal:  PLoS One       Date:  2014-12-17       Impact factor: 3.240

2.  Use of serology and real time PCR to control an outbreak of bovine brucellosis at a dairy cattle farm in the Nile Delta region, Egypt.

Authors:  Mayada Gwida; Maged El-Ashker; Falk Melzer; Mohamed El-Diasty; Mohamed El-Beskawy; Heinrich Neubauer
Journal:  Ir Vet J       Date:  2016-02-24       Impact factor: 2.146

3.  Epidemiology of Brucellosis and Genetic Diversity of Brucella abortus in Kazakhstan.

Authors:  Elena Shevtsova; Alexandr Shevtsov; Kasim Mukanov; Maxim Filipenko; Dinara Kamalova; Igor Sytnik; Marat Syzdykov; Andrey Kuznetsov; Assel Akhmetova; Mira Zharova; Talgat Karibaev; Pavel Tarlykov; Erlan Ramanculov
Journal:  PLoS One       Date:  2016-12-01       Impact factor: 3.240

4.  Origins and global context of Brucella abortus in Italy.

Authors:  Giuliano Garofolo; Elisabetta Di Giannatale; Ilenia Platone; Katiuscia Zilli; Lorena Sacchini; Anna Abass; Massimo Ancora; Cesare Cammà; Guido Di Donato; Fabrizio De Massis; Paolo Calistri; Kevin P Drees; Jeffrey T Foster
Journal:  BMC Microbiol       Date:  2017-02-02       Impact factor: 3.605

5.  Rapid and safe one-step extraction method for the identification of Brucella strains at genus and species level by MALDI-TOF mass spectrometry.

Authors:  Michela Sali; Flavio De Maio; Michela Tarantino; Giuliano Garofolo; Manuela Tittarelli; Lorena Sacchini; Katiuscia Zilli; Paolo Pasquali; Paola Petrucci; Cinzia Marianelli; Massimiliano Francia; Maurizio Sanguinetti; Rosanna Adone
Journal:  PLoS One       Date:  2018-06-05       Impact factor: 3.240

6.  Brucella melitensis in France: persistence in wildlife and probable spillover from Alpine ibex to domestic animals.

Authors:  Virginie Mick; Gilles Le Carrou; Yannick Corde; Yvette Game; Maryne Jay; Bruno Garin-Bastuji
Journal:  PLoS One       Date:  2014-04-14       Impact factor: 3.240

7.  Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients.

Authors:  Subok Lee; Kyu-Jam Hwang; Mi-Yeoun Park; Seon-Do Hwang; Hee-Youl Chai; Hyuk Chu; Sang-Hee Park
Journal:  Osong Public Health Res Perspect       Date:  2013-10

8.  Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis.

Authors:  Ahmed M S Menshawy; Marta Perez-Sancho; Teresa Garcia-Seco; Hosein I Hosein; Nerea García; Irene Martinez; Ashraf E Sayour; Joaquín Goyache; Ragab A A Azzam; Lucas Dominguez; Julio Alvarez
Journal:  Biomed Res Int       Date:  2014-01-06       Impact factor: 3.411

Review 9.  Polymerase chain reaction-based assays for the diagnosis of human brucellosis.

Authors:  Ying Wang; Zhanli Wang; Yaxian Zhang; Liyun Bai; Yue Zhao; Chunfang Liu; An Ma; Hui Yu
Journal:  Ann Clin Microbiol Antimicrob       Date:  2014-08-01       Impact factor: 3.944

10.  Cases of human brucellosis in Sweden linked to Middle East and Africa.

Authors:  Giuliano Garofolo; Antonio Fasanella; Elisabetta Di Giannatale; Ilenia Platone; Lorena Sacchini; Tiziana Persiani; Talar Boskani; Kristina Rizzardi; Tara Wahab
Journal:  BMC Res Notes       Date:  2016-05-17
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