Literature DB >> 23160137

Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens.

Javier Rodríguez-Moya1, Montserrat Argandoña, Fernando Iglesias-Guerra, Joaquín J Nieto, Carmen Vargas.   

Abstract

Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.

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Year:  2012        PMID： 23160137      PMCID： PMC3568561          DOI： 10.1128/AEM.02774-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  23 in total

1.  High-yield cultivation of Marinococcus M52 for production and recovery of hydroxyectoine.

Authors:  Chiara Schiraldi; Carmelina Maresca; Angela Catapano; Erwin A Galinski; Mario De Rosa
Journal:  Res Microbiol       Date:  2006-04-04       Impact factor: 3.992

2.  Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in Escherichia coli.

Authors:  Petra Louis; Erwin A Galinski
Journal:  Microbiology (Reading)       Date:  1997-04       Impact factor: 2.777

3.  Natural and engineered hydroxyectoine production based on the Pseudomonas stutzeri ectABCD-ask gene cluster.

Authors:  Britta Seip; Erwin A Galinski; Matthias Kurz
Journal:  Appl Environ Microbiol       Date:  2010-12-17       Impact factor: 4.792

4.  High productivity of ectoines by Halomonas boliviensis using a combined two-step fed-batch culture and milking process.

Authors:  Doan Van-Thuoc; Héctor Guzmán; Jorge Quillaguamán; Rajni Hatti-Kaul
Journal:  J Biotechnol       Date:  2010-03-17       Impact factor: 3.307

5.  Osmoprotectants in Halomonas elongata: high-affinity betaine transport system and choline-betaine pathway.

Authors:  D Cánovas; C Vargas; L N Csonka; A Ventosa; J J Nieto
Journal:  J Bacteriol       Date:  1996-12       Impact factor: 3.490

6.  Isolation of cryptic plasmids from moderately halophilic eubacteria of the genus Halomonas. Characterization of a small plasmid from H. elongata and its use for shuttle vector construction.

Authors:  C Vargas; R Fernández-Castillo; D Cánovas; A Ventosa; J J Nieto
Journal:  Mol Gen Genet       Date:  1995-02-20

7.  Functional expression of the ectoine hydroxylase gene (thpD) from Streptomyces chrysomallus in Halomonas elongata.

Authors:  Julia Prabhu; Florian Schauwecker; Nicolas Grammel; Ullrich Keller; Michael Bernhard
Journal:  Appl Environ Microbiol       Date:  2004-05       Impact factor: 4.792

8.  Role of trehalose in salinity and temperature tolerance in the model halophilic bacterium Chromohalobacter salexigens.

Authors:  Mercedes Reina-Bueno; Montserrat Argandoña; Manuel Salvador; Javier Rodríguez-Moya; Fernando Iglesias-Guerra; Laszlo N Csonka; Joaquín J Nieto; Carmen Vargas
Journal:  PLoS One       Date:  2012-03-20       Impact factor: 3.240

9.  A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581 T.

Authors:  Karin Schwibbert; Alberto Marin-Sanguino; Irina Bagyan; Gabriele Heidrich; Georg Lentzen; Harald Seitz; Markus Rampp; Stephan C Schuster; Hans-Peter Klenk; Friedhelm Pfeiffer; Dieter Oesterhelt; Hans Jörg Kunte
Journal:  Environ Microbiol       Date:  2010-09-16       Impact factor: 5.491

10.  Unravelling the adaptation responses to osmotic and temperature stress in Chromohalobacter salexigens, a bacterium with broad salinity tolerance.

Authors:  Carmen Vargas; Montserrat Argandoña; Mercedes Reina-Bueno; Javier Rodríguez-Moya; Cristina Fernández-Aunión; Joaquín J Nieto
Journal:  Saline Systems       Date:  2008-09-15
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  14 in total

1.  The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum.

Authors:  Katharina D Moritz; Birgit Amendt; Elisabeth M H J Witt; Erwin A Galinski
Journal:  Extremophiles       Date:  2014-08-21       Impact factor: 2.395

Review 2.  Role of the Extremolytes Ectoine and Hydroxyectoine as Stress Protectants and Nutrients: Genetics, Phylogenomics, Biochemistry, and Structural Analysis.

Authors:  Laura Czech; Lucas Hermann; Nadine Stöveken; Alexandra A Richter; Astrid Höppner; Sander H J Smits; Johann Heider; Erhard Bremer
Journal:  Genes (Basel)       Date:  2018-03-22       Impact factor: 4.096

3.  Accumulation of Ectoines By Halophilic Bacteria Isolated from Fermented Shrimp Paste: An Adaptation Mechanism to Salinity, Temperature, and pH Stress.

Authors:  Doan Van Thuoc; Tran Thi Loan; Nguyen Thi Tra
Journal:  Curr Microbiol       Date:  2021-04-08       Impact factor: 2.188

4.  Tinkering with Osmotically Controlled Transcription Allows Enhanced Production and Excretion of Ectoine and Hydroxyectoine from a Microbial Cell Factory.

Authors:  Laura Czech; Sebastian Poehl; Philipp Hub; Nadine Stöveken; Erhard Bremer
Journal:  Appl Environ Microbiol       Date:  2018-01-02       Impact factor: 4.792

5.  Biochemical properties of ectoine hydroxylases from extremophiles and their wider taxonomic distribution among microorganisms.

Authors:  Nils Widderich; Astrid Höppner; Marco Pittelkow; Johann Heider; Sander H J Smits; Erhard Bremer
Journal:  PLoS One       Date:  2014-04-08       Impact factor: 3.240

6.  EctD-mediated biotransformation of the chemical chaperone ectoine into hydroxyectoine and its mechanosensitive channel-independent excretion.

Authors:  Laura Czech; Nadine Stöveken; Erhard Bremer
Journal:  Microb Cell Fact       Date:  2016-07-20       Impact factor: 5.328

7.  Understanding the interplay of carbon and nitrogen supply for ectoines production and metabolic overflow in high density cultures of Chromohalobacter salexigens.

Authors:  María J Salar-García; Vicente Bernal; José M Pastor; Manuel Salvador; Montserrat Argandoña; Joaquín J Nieto; Carmen Vargas; Manuel Cánovas
Journal:  Microb Cell Fact       Date:  2017-02-08       Impact factor: 5.328

8.  Insights into metabolic osmoadaptation of the ectoines-producer bacterium Chromohalobacter salexigens through a high-quality genome scale metabolic model.

Authors:  Francine Piubeli; Manuel Salvador; Montserrat Argandoña; Joaquín J Nieto; Vicente Bernal; Jose M Pastor; Manuel Cánovas; Carmen Vargas
Journal:  Microb Cell Fact       Date:  2018-01-09       Impact factor: 5.328

9.  Glass-forming property of hydroxyectoine is the cause of its superior function as a desiccation protectant.

Authors:  Christoph Tanne; Elena A Golovina; Folkert A Hoekstra; Andrea Meffert; Erwin A Galinski
Journal:  Front Microbiol       Date:  2014-04-04       Impact factor: 5.640

10.  Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine.

Authors:  Judith Becker; Rudolf Schäfer; Michael Kohlstedt; Björn J Harder; Nicole S Borchert; Nadine Stöveken; Erhard Bremer; Christoph Wittmann
Journal:  Microb Cell Fact       Date:  2013-11-15       Impact factor: 5.328
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