Literature DB >> 23151786

Efficient multi-site-directed mutagenesis directly from genomic template.

Fengtao Luo1, Xiaolan Du, Tujun Weng, Xuan Wen, Junlan Huang, Lin Chen.   

Abstract

In this article, the traditional multi-site-directed mutagenesis method based on overlap extension PCR was improved specifically for complicated templates, such as genomic sequence or complementary DNA. This method was effectively applied for multi-site-directed mutagenesis directly from mouse genomic DNA, as well as for combination, deletion or insertion of DNA fragments.

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Year:  2012        PMID： 23151786     DOI： 10.1007/s12038-012-9257-8

Source DB:  PubMed          Journal:  J Biosci        ISSN： 0250-5991            Impact factor:   1.826


  9 in total

1.  Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis.

Authors:  W Wang; B A Malcolm
Journal:  Biotechniques       Date:  1999-04       Impact factor: 1.993

2.  Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit.

Authors:  Holly H Hogrefe; Janice Cline; Geri L Youngblood; Ronda M Allen
Journal:  Biotechniques       Date:  2002-11       Impact factor: 1.993

3.  Determination of the secondary structure of and cellular protein binding to the 3'-untranslated region of the hepatitis C virus RNA genome.

Authors:  T Ito; M M Lai
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103

4.  A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR.

Authors:  Yingfeng An; Jianfei Ji; Wenfang Wu; Anguo Lv; Ribo Huang; Yutuo Wei
Journal:  Appl Microbiol Biotechnol       Date:  2005-10-13       Impact factor: 4.813

5.  A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis.

Authors:  Ri-He Peng; Ai-Sheng Xiong; Quan-Hong Yao
Journal:  Appl Microbiol Biotechnol       Date:  2006-10-05       Impact factor: 4.813

6.  Asymmetric overlap extension PCR method bypassing intermediate purification and the amplification of wild-type template in site-directed mutagenesis.

Authors:  Yue-Hua Xiao; Meng-Hui Yin; Lei Hou; Ming Luo; Yan Pei
Journal:  Biotechnol Lett       Date:  2007-03-14       Impact factor: 2.461

Review 7.  Approaches to DNA mutagenesis: an overview.

Authors:  M M Ling; B H Robinson
Journal:  Anal Biochem       Date:  1997-12-15       Impact factor: 3.365

8.  A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.

Authors:  R Higuchi; B Krummel; R K Saiki
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

9.  A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step.

Authors:  Rajiv Tyagi; Richard Lai; Ronald G Duggleby
Journal:  BMC Biotechnol       Date:  2004-02-26       Impact factor: 2.563
  9 in total
  5 in total

1.  MicroRNA-433 regulates apoptosis by targeting PDCD4 in human osteosarcoma cells.

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Journal:  Oncol Lett       Date:  2017-06-21       Impact factor: 2.967

Review 2.  Perspective on Adeno-Associated Virus Capsid Modification for Duchenne Muscular Dystrophy Gene Therapy.

Authors:  Michael E Nance; Dongsheng Duan
Journal:  Hum Gene Ther       Date:  2015-10-15       Impact factor: 5.695

Review 3.  Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression.

Authors:  Jian-zhong Xu; Wei-guo Zhang
Journal:  J Zhejiang Univ Sci B       Date:  2016-02       Impact factor: 3.066

4.  Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis.

Authors:  Hasnain Hussain; Nikson Fatt-Ming Chong
Journal:  Biomed Res Int       Date:  2016-11-22       Impact factor: 3.411

5.  IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly.

Authors:  Javier García-Nafría; Jake F Watson; Ingo H Greger
Journal:  Sci Rep       Date:  2016-06-06       Impact factor: 4.379
  5 in total

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