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Literature DB >> 23145435

Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G.

Junya Chiba¹, Takahide Kouno, Shun Aoki, Hitoshi Sato, JingYing Zhang, Hiroshi Matsuo, Masahiko Inouye.

Abstract

APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.

Entities: Chemical Disease Gene Species

Mesh：

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Year: 2012 PMID： 23145435 PMCID： PMC3541039 DOI： 10.1039/c2cc36779c

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

23 in total

1. An anthropoid-specific locus of orphan C to U RNA-editing enzymes on chromosome 22.

Authors: Adam Jarmuz; Ann Chester; Jayne Bayliss; Jane Gisbourne; Ian Dunham; James Scott; Naveenan Navaratnam
Journal: Genomics Date: 2002-03 Impact factor: 5.736

2. 3'-Ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces: novel model systems for exploring flexibility of short DNA using cyclic voltammetry.

Authors: Agnès Anne; Agnès Bouchardon; Jacques Moiroux
Journal: J Am Chem Soc Date: 2003-02-05 Impact factor: 15.419

3. DNA deamination mediates innate immunity to retroviral infection.

Authors: Reuben S Harris; Kate N Bishop; Ann M Sheehy; Heather M Craig; Svend K Petersen-Mahrt; Ian N Watt; Michael S Neuberger; Michael H Malim
Journal: Cell Date: 2003-06-13 Impact factor: 41.582

4. Electrochemical detection of insertion/deletion mutations based on enhanced flexibility of bulge-containing duplexes on electrodes.

Authors: Junya Chiba; Ayumi Akaishi; Reona Ikeda; Masahiko Inouye
Journal: Chem Commun (Camb) Date: 2010-09-17 Impact factor: 6.222

5. Electrochemical genotyping by using two ferrocene/isoquinoline-connected DNA probes with different redox potentials on a single electrode.

Authors: Reona Ikeda; Satoshi Kitagawa; Junya Chiba; Masahiko Inouye
Journal: Chemistry Date: 2009-07-20 Impact factor: 5.236

Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G.

1. An anthropoid-specific locus of orphan C to U RNA-editing enzymes on chromosome 22.

2. 3'-Ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces: novel model systems for exploring flexibility of short DNA using cyclic voltammetry.

3. DNA deamination mediates innate immunity to retroviral infection.

4. Electrochemical detection of insertion/deletion mutations based on enhanced flexibility of bulge-containing duplexes on electrodes.

5. Electrochemical genotyping by using two ferrocene/isoquinoline-connected DNA probes with different redox potentials on a single electrode.

6. A sensitive signal-on electrochemical assay for MTase activity using AuNPs amplification.

7. An electrochemical approach for detection of DNA methylation and assay of the methyltransferase activity.

8. Electrochemical DNA methylation detection for enzymatically digested CpG oligonucleotides.

9. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein.

Review 10. Interactions of host APOBEC3 restriction factors with HIV-1 in vivo: implications for therapeutics.

1. Mechanism for APOBEC3G catalytic exclusion of RNA and non-substrate DNA.

2. Impact of H216 on the DNA binding and catalytic activities of the HIV restriction factor APOBEC3G.

3. Crystal structure of the catalytic domain of HIV-1 restriction factor APOBEC3G in complex with ssDNA.