CONTEXT: It is recommended to measure thyroglobulin (Tg) levels in the needle washout fluids from fine-needle aspirations (FNAs) in patients with papillary thyroid carcinoma (PTC) who have ultrasonographically suspicious metastatic lymph nodes (LNs). However, it is not clear whether serum anti-Tg antibodies (TgAbs) interfere with the detection of Tg in needle washout fluids from FNAs (FNA-Tg). OBJECTIVE: The objective of the study was to evaluate the influence of serum TgAbs on FNA-Tg detection. DESIGN AND SETTINGS: This retrospective observational cohort study enrolled 207 patients with conventional PTC in whom FNA-Tg values had been measured. All patients initially underwent total thyroidectomy and remnant ablation. FNA-Tg levels were measured from ultrasonographically suspicious metastatic LNs of 0.5 cm or greater in the longest diameter. RESULTS: From 207 patients, 263 LNs were evaluated. Final histopathology was available for 92 LNs, of which 88 (96%) were malignant. FNA-Tg levels were lower in the LNs from serum TgAb-positive patients than in those from TgAb-negative patients (P < 0.001). In four of 13 metastatic LNs from TgAb-positive patients, the FNA-Tg levels were below 10 μg/liter including one in which both FNA-Tg and serum-stimulated Tg levels were below 1 μg/liter and stained positively for Tg in pathology. There was also one malignant LN with negative for FNA-Tg, serum-stimulated Tg, and serum TgAb but that nonetheless stained intensely for Tg. However, there were no malignant LNs with both negative cytology and negative FNA-Tg. A diagnosis based on FNA-Tg had a lower sensitivity and negative predictive value in the TgAb-positive group than in the TgAb-negative group. CONCLUSION: FNA-Tg measurement is highly reliable in the diagnosis of neck metastases in PTC patients, even in cases of negative-stimulated Tg or positive TgAb. However, high-serum TgAb levels could interfere with FNA-Tg measurements and thereby result in falsely low FNA-Tg levels.
CONTEXT: It is recommended to measure thyroglobulin (Tg) levels in the needle washout fluids from fine-needle aspirations (FNAs) in patients with papillary thyroid carcinoma (PTC) who have ultrasonographically suspicious metastatic lymph nodes (LNs). However, it is not clear whether serum anti-Tg antibodies (TgAbs) interfere with the detection of Tg in needle washout fluids from FNAs (FNA-Tg). OBJECTIVE: The objective of the study was to evaluate the influence of serum TgAbs on FNA-Tg detection. DESIGN AND SETTINGS: This retrospective observational cohort study enrolled 207 patients with conventional PTC in whom FNA-Tg values had been measured. All patients initially underwent total thyroidectomy and remnant ablation. FNA-Tg levels were measured from ultrasonographically suspicious metastatic LNs of 0.5 cm or greater in the longest diameter. RESULTS: From 207 patients, 263 LNs were evaluated. Final histopathology was available for 92 LNs, of which 88 (96%) were malignant. FNA-Tg levels were lower in the LNs from serum TgAb-positive patients than in those from TgAb-negative patients (P < 0.001). In four of 13 metastatic LNs from TgAb-positive patients, the FNA-Tg levels were below 10 μg/liter including one in which both FNA-Tg and serum-stimulated Tg levels were below 1 μg/liter and stained positively for Tg in pathology. There was also one malignant LN with negative for FNA-Tg, serum-stimulated Tg, and serum TgAb but that nonetheless stained intensely for Tg. However, there were no malignant LNs with both negative cytology and negative FNA-Tg. A diagnosis based on FNA-Tg had a lower sensitivity and negative predictive value in the TgAb-positive group than in the TgAb-negative group. CONCLUSION: FNA-Tg measurement is highly reliable in the diagnosis of neck metastases in PTC patients, even in cases of negative-stimulated Tg or positive TgAb. However, high-serum TgAb levels could interfere with FNA-Tg measurements and thereby result in falsely low FNA-Tg levels.
Authors: Mijin Kim; Tae Yong Kim; Soo Han Kim; Yunkyoung Lee; Su-yeon Park; Hyung-Don Kim; Hyemi Kwon; Yun Mi Choi; Eun Kyung Jang; Min Ji Jeon; Won Gu Kim; Young Kee Shong; Won Bae Kim Journal: Korean J Intern Med Date: 2015-04-29 Impact factor: 2.884
Authors: Bryan R Haugen; Erik K Alexander; Keith C Bible; Gerard M Doherty; Susan J Mandel; Yuri E Nikiforov; Furio Pacini; Gregory W Randolph; Anna M Sawka; Martin Schlumberger; Kathryn G Schuff; Steven I Sherman; Julie Ann Sosa; David L Steward; R Michael Tuttle; Leonard Wartofsky Journal: Thyroid Date: 2016-01 Impact factor: 6.568
Authors: Youn Hee Jee; Francesco S Celi; Maureen Sampson; David B Sacks; Alan T Remaley; Electron Kebebew; Jeffrey Baron Journal: Clin Endocrinol (Oxf) Date: 2015-01-15 Impact factor: 3.478
Authors: Marta Amaro da Silveira Duval; André Borsatto Zanella; Ana Patrícia Cristo; Carlo Sasso Faccin; Marcia Silva Graudenz; Ana Luiza Maia Journal: Eur Thyroid J Date: 2017-09-06
Authors: Ivan Blažeković; Matija Romić; Marija Bosak Butković; Ines Šiško Markoš; Vedrana Gladić Nenadić; Valentina Vidranski; Petra Petranović Ovčariček; Dražena Krilić; Tea Varjačić; Josip Staničić; Roko Granić; Marija Punda; Ivan Mihaljević; Tomislav Jukić; Maja Franceschi Journal: Acta Clin Croat Date: 2020-06 Impact factor: 0.780