Hanwen Sun1, Hongliu Wang, Xusheng Ge. 1. College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding, P.R. China. hanwen@hbu.edu.cn
Abstract
AIM: To develop a new high-performance liquid chromatography (HPLC) method for simultaneous determination of the combined drugs (ceftriaxone sodium, metronidazole, and levofloxacin) in human urine. METHODS: Ceftriaxone sodium, metronidazole, and levofloxacin were separated on a Kromasil 100-5 C18 (250 mm × 4.6 mm, 5 μm, AKZO NOBEL, Bohus, Sweden) analytical column, using the mobile phase consisted of 1.5 mM KH(2) PO(4) (pH 4.5) with 0.0125% triethylamine-methnol (70:30, v/v). Ceftriaxone sodium, metronidazole, and levofloxacin were detected by a photodiode-array detector at 247, 320, 292 nm, respectively. RESULTS: Under optimal conditions, the effective separation of ceftriaxone sodium, metronidazole, and levofloxacin was achieved. A good linearity with the correlation coefficients more than 0.999 was demonstrated. The detection limits of ceftriaxone sodium, metronidazole, and levofloxacin were 0.05, 0.01, and 0.25 μg/ml, respectively, and the average recoveries in human urine were in the range from 97.73 to 100.7% with the average relative standard deviation (RSD) in the range of 2.5% and 3.0%. CONCLUSION: The proposed method was sensitive, accurate, and rapid. This work may provide a reference for clinical rational drug use and methodology for the pharmacokinetics study of the combined drugs.
AIM: To develop a new high-performance liquid chromatography (HPLC) method for simultaneous determination of the combined drugs (ceftriaxone sodium, metronidazole, and levofloxacin) in human urine. METHODS:Ceftriaxone sodium, metronidazole, and levofloxacin were separated on a Kromasil 100-5 C18 (250 mm × 4.6 mm, 5 μm, AKZO NOBEL, Bohus, Sweden) analytical column, using the mobile phase consisted of 1.5 mM KH(2) PO(4) (pH 4.5) with 0.0125% triethylamine-methnol (70:30, v/v). Ceftriaxone sodium, metronidazole, and levofloxacin were detected by a photodiode-array detector at 247, 320, 292 nm, respectively. RESULTS: Under optimal conditions, the effective separation of ceftriaxone sodium, metronidazole, and levofloxacin was achieved. A good linearity with the correlation coefficients more than 0.999 was demonstrated. The detection limits of ceftriaxone sodium, metronidazole, and levofloxacin were 0.05, 0.01, and 0.25 μg/ml, respectively, and the average recoveries in human urine were in the range from 97.73 to 100.7% with the average relative standard deviation (RSD) in the range of 2.5% and 3.0%. CONCLUSION: The proposed method was sensitive, accurate, and rapid. This work may provide a reference for clinical rational drug use and methodology for the pharmacokinetics study of the combined drugs.
Authors: S Djabarouti; E Boselli; B Allaouchiche; B Ba; A T Nguyen; J B Gordien; J M Bernadou; M C Saux; D Breilh Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2004-01-05 Impact factor: 3.205
Authors: M J Galmier; A M Frasey; M Bastide; E Beyssac; J Petit; J M Aiache; C Lartigue-Mattei Journal: J Chromatogr B Biomed Sci Appl Date: 1998-12-11
Authors: Marcelle O Koeppe; Rodrigo Cristofoletti; Eduardo F Fernandes; Silvia Storpirtis; Hans E Junginger; Sabine Kopp; Kamal K Midha; Vinod P Shah; Salomon Stavchansky; Jennifer B Dressman; Dirk M Barends Journal: J Pharm Sci Date: 2011-01-21 Impact factor: 3.534
Authors: Brett C McWhinney; Steven C Wallis; Tara Hillister; Jason A Roberts; Jeffrey Lipman; Jacobus P J Ungerer Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2010-05-24 Impact factor: 3.205
Authors: Hadir M Maher; Rasha M Youssef; Riad H Khalil; Sabry M El-Bahr Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2008-11-01 Impact factor: 3.205