Kirtimaan Syal1, Anand Srinivasan, Dibyajyoti Banerjee. 1. Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.
Abstract
OBJECTIVES: To study the potential of commonly used aminoglycoside antibiotics to form non-creatinine chromogen with alkaline picrate reagent. DESIGN AND METHODS: We studied the non-creatinine chromogen formation of various concentrations of streptomycin, amikacin, kanamycin, netilmicin, gentamicin and tobramycin added to known creatinine concentrations by the Jaffe reaction based creatinine estimation. RESULTS: Only streptomycin above therapeutic concentrations of 10mg/mL interfered in the Jaffe reaction and acted as non-creatinine chromogen. CONCLUSIONS: Therapeutic doses of the aminoglycosides do not form non-creatinine chromogens.
OBJECTIVES: To study the potential of commonly used aminoglycoside antibiotics to form non-creatinine chromogen with alkaline picrate reagent. DESIGN AND METHODS: We studied the non-creatinine chromogen formation of various concentrations of streptomycin, amikacin, kanamycin, netilmicin, gentamicin and tobramycin added to known creatinine concentrations by the Jaffe reaction based creatinine estimation. RESULTS: Only streptomycin above therapeutic concentrations of 10mg/mL interfered in the Jaffe reaction and acted as non-creatinine chromogen. CONCLUSIONS: Therapeutic doses of the aminoglycosides do not form non-creatinine chromogens.
Authors: Matthew T James; Charles E Hobson; Michael Darmon; Sumit Mohan; Darren Hudson; Stuart L Goldstein; Claudio Ronco; John A Kellum; Sean M Bagshaw Journal: Can J Kidney Health Dis Date: 2016-02-26