Literature DB >> 23104332

Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun).

Anssi L Vuorinen1, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, Jari P T Valkonen.   

Abstract

Non-vacuum gene guns such as HandyGun are flexible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation efficiency of plant cells with plasmid DNA using HandyGun will be provided.

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Year:  2013        PMID: 23104332     DOI: 10.1007/978-1-62703-110-3_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  1 in total

1.  An improved biolistic delivery and analysis method for evaluation of DNA and CRISPR-Cas delivery efficacy in plant tissue.

Authors:  Kyle Miller; Alan L Eggenberger; Keunsub Lee; Fei Liu; Minjeong Kang; Madison Drent; Andrew Ruba; Tyler Kirscht; Kan Wang; Shan Jiang
Journal:  Sci Rep       Date:  2021-04-08       Impact factor: 4.379

  1 in total

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