| Literature DB >> 23093669 |
Joel Marh1, Zoia Stoytcheva, Johann Urschitz, Atsushi Sugawara, Hideaki Yamashiro, Jesse B Owens, Ilko Stoytchev, Pawel Pelczar, Ryuzo Yanagimachi, Stefan Moisyadi.
Abstract
We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse genome. This procedure increased transgene integration efficiency by fivefold compared with conventional PNI or intracytoplasmic sperm injection-mediated transgenesis. Our data indicate that the transposase-enhanced PNI technique additionally requires fewer embryos to be microinjected than traditional methods to obtain transgenic animals. This transposase-mediated approach is also very efficient for single-cell embryo cytoplasmic injections, offering an easy-to-implement transgenesis method to the scientific community.Entities:
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Year: 2012 PMID： 23093669 PMCID： PMC3511126 DOI： 10.1073/pnas.1216473109
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205