Literature DB >> 23086339

Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.

Elodie Barbau-Piednoir1, Nadine Botteldoorn, Marc Yde, Jacques Mahillon, Nancy H Roosens.   

Abstract

A combination of four qualitative SYBR®Green qPCR screening assays targeting two levels of discrimination: Listeria genus (except Listeria grayi) and Listeria monocytogenes, is presented. These assays have been developed to be run simultaneously using the same polymerase chain reaction (PCR) programme. The paper also proposes a new validation procedure to specifically validate qPCR assays applied to food microbiology according to two guidelines: the ISO 22118 norm and the "Definition of minimum performance requirements for analytical methods of GMO testing". The developed assays target the iap, prs and hlyA genes that belong to or neighbour the virulence cluster of Listeria spp. The selected primers were designed to amplify short fragments (60 to 103 bp) in order to obtain optimal PCR efficiency (between 97 and 107 % efficiency). The limit of detection of the SYBR®Green qPCR assays is two to five copies of target genes per qPCR reaction. These assays are highly accurate (98.08 and 100 % accuracy for the Listeria spp. and L. monocytogenes assays, respectively).

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Year:  2012        PMID： 23086339      PMCID： PMC3627020          DOI： 10.1007/s00253-012-4477-2

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  38 in total

1.  Incidence and control of Listeria monocytogenes in foods in Denmark.

Authors:  B Nørrung; J K Andersen; J Schlundt
Journal:  Int J Food Microbiol       Date:  1999-12-15       Impact factor: 5.277

2.  Detection and quantification of Listeria monocytogenes by 5'-nuclease polymerase chain reaction targeting the actA gene.

Authors:  K Oravcová; E Kaclíková; K Krascsenicsová; D Pangallo; B Brezná; P Siekel; T Kuchta
Journal:  Lett Appl Microbiol       Date:  2006-01       Impact factor: 2.858

3.  Exposure of Listeria monocytogenes within an epidemic caused by butter in Finland.

Authors:  R Maijala; O Lyytikäinen; T Autio; T Aalto; L Haavisto; T Honkanen-Buzalski
Journal:  Int J Food Microbiol       Date:  2001-10-22       Impact factor: 5.277

4.  Detection of Listeria monocytogenes in food using a combined enrichment/real-time PCR method targeting the prfA gene.

Authors:  Peter Rossmanith; Martina Krassnig; Martin Wagner; Ingeborg Hein
Journal:  Res Microbiol       Date:  2006-04-03       Impact factor: 3.992

Review 5.  Recent advances in quantitative PCR (qPCR) applications in food microbiology.

Authors:  Florence Postollec; Hélène Falentin; Sonia Pavan; Jérôme Combrisson; Danièle Sohier
Journal:  Food Microbiol       Date:  2011-04-01       Impact factor: 5.516

6.  [Acute purulent Listeria seelingeri meningitis in an immunocompetent adult].

Authors:  J Rocourt; H Hof; A Schrettenbrunner; R Malinverni; J Bille
Journal:  Schweiz Med Wochenschr       Date:  1986-02-22

Review 7.  Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review.

Authors:  Uta Gasanov; Denise Hughes; Philip M Hansbro
Journal:  FEMS Microbiol Rev       Date:  2004-12-22       Impact factor: 16.408

8.  Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target.

Authors:  Justin O' Grady; Sara Sedano-Balbás; Majella Maher; Terry Smith; Thomas Barry
Journal:  Food Microbiol       Date:  2007-08-06       Impact factor: 5.516

9.  Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR.

Authors:  P G Klein; V K Juneja
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792

10.  Use of PCR primers derived from a putative transcriptional regulator gene for species-specific determination of Listeria monocytogenes.

Authors:  Dongyou Liu; A Jerald Ainsworth; Frank W Austin; Mark L Lawrence
Journal:  Int J Food Microbiol       Date:  2004-03-15       Impact factor: 5.277
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  14 in total

1.  p53 Hinders CRISPR/Cas9-Mediated Targeted Gene Disruption in Memory CD8 T Cells In Vivo.

Authors:  Samarchith P Kurup; Steven J Moioffer; Lecia L Pewe; John T Harty
Journal:  J Immunol       Date:  2020-09-04       Impact factor: 5.422

2.  Mining of novel species-specific primers for PCR detection of Listeria monocytogenes based on genomic approach.

Authors:  Tingting Tao; Qiming Chen; Xiaomei Bie; Fengxia Lu; Zhaoxin Lu
Journal:  World J Microbiol Biotechnol       Date:  2015-09-09       Impact factor: 3.312

3.  Prevalence, characterization and antimicrobial resistance of Listeria monocytogenes isolated from beef meat in Meknes city, Morocco.

Authors:  Meryem Boukili; Fouzia Rhazi Filali; Nada Lafkih; Aziz Bouymajane; Majida Sefiani; Mohieddine Moumni
Journal:  Germs       Date:  2020-06-02

4.  A new rapid method for detecting epidermal growth factor receptor mutations in non-small cell lung cancer.

Authors:  Miyako Takata; Hiroki Chikumi; Keiji Matsunami; Masahiro Kodani; Tomohiro Sakamoto; Kazuhiro Hashimoto; Masaki Nakamoto; Kensaku Okada; Tsuyoshi Kitaura; Shingo Matsumoto; Jun Kurai; Akira Yamasaki; Tadashi Igishi; Naoto Burioka; Eiji Shimizu
Journal:  Oncol Rep       Date:  2015-01-14       Impact factor: 3.906

5.  Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Authors:  X Libert; C Chasseur; S Bladt; A Packeu; F Bureau; N H Roosens; S C J De Keersmaecker
Journal:  Appl Microbiol Biotechnol       Date:  2015-07-17       Impact factor: 4.813

6.  SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.

Authors:  Elodie Barbau-Piednoir; Sophie Bertrand; Jacques Mahillon; Nancy H Roosens; Nadine Botteldoorn
Journal:  Appl Microbiol Biotechnol       Date:  2013-10-11       Impact factor: 4.813

7.  Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

Authors:  Xavier Libert; Ann Packeu; Fabrice Bureau; Nancy H Roosens; Sigrid C J De Keersmaecker
Journal:  PLoS One       Date:  2017-03-09       Impact factor: 3.240

8.  A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay.

Authors:  X Libert; C Chasseur; A Packeu; F Bureau; N H Roosens; S J C De Keersmaecker
Journal:  Appl Microbiol Biotechnol       Date:  2015-11-28       Impact factor: 4.813

Review 9.  An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food.

Authors:  Jodi Woan-Fei Law; Nurul-Syakima Ab Mutalib; Kok-Gan Chan; Learn-Han Lee
Journal:  Front Microbiol       Date:  2015-11-03       Impact factor: 5.640

10.  Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.

Authors:  Elodie Barbau-Piednoir; Sigrid C J De Keersmaecker; Maud Delvoye; Céline Gau; Patrick Philipp; Nancy H Roosens
Journal:  BMC Biotechnol       Date:  2015-11-11       Impact factor: 2.563
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