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Literature DB >> 23085614

Membrane-protein binding measured with solution-phase plasmonic nanocube sensors.

Hung-Jen Wu¹, Joel Henzie, Wan-Chen Lin, Christopher Rhodes, Zhu Li, Elodie Sartorel, Jeremy Thorner, Peidong Yang, Jay T Groves.

Abstract

We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.

Entities: Chemical

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Year: 2012 PMID： 23085614 PMCID： PMC3703907 DOI： 10.1038/nmeth.2211

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

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