Literature DB >> 23065615

Fast and easy method for construction of plasmid vectors using modified quick-change mutagenesis.

Jin Woo Bok1, Nancy P Keller.   

Abstract

Plasmid vector construction is an essential step for molecular microbiology yet often a time-consuming process. Manipulation of the fungal genome to express genes to activate secondary metabolite production often requires creation of plasmid constructs in a reiterative fashion. Here we introduce a modified Quick-change site-directed mutagenesis method that allows for rapid and accurate construction of fungal transformation vectors.

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Year:  2012        PMID： 23065615      PMCID： PMC3692276          DOI： 10.1007/978-1-62703-122-6_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  20 in total

1.  Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase.

Authors:  M Geiser; R Cèbe; D Drewello; R Schmitz
Journal:  Biotechniques       Date:  2001-07       Impact factor: 1.993

2.  Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round.

Authors:  Andreas Seyfang; Jean Huaqian Jin
Journal:  Anal Biochem       Date:  2004-01-15       Impact factor: 3.365

3.  Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes.

Authors:  M Nelson; M McClelland
Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

Review 4.  Recent advances in the polymerase chain reaction.

Authors:  H A Erlich; D Gelfand; J J Sninsky
Journal:  Science       Date:  1991-06-21       Impact factor: 47.728

5.  Preferential ligation during TA-cloning of multitemplate PCR products--a factor causing bias in microbial community structure analysis.

Authors:  Márton Palatinszky; Marcell Nikolausz; Domonkos Sváb; Károly Márialigeti
Journal:  J Microbiol Methods       Date:  2011-02-18       Impact factor: 2.363

6.  A simple and rapid method for the selection of oligodeoxynucleotide-directed mutants.

Authors:  M A Vandeyar; M P Weiner; C J Hutton; C A Batt
Journal:  Gene       Date:  1988-05-15       Impact factor: 3.688

7.  Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease.

Authors:  G E Geier; P Modrich
Journal:  J Biol Chem       Date:  1979-02-25       Impact factor: 5.157

Review 8.  Bacterial plasmids: their extraordinary contribution to molecular genetics.

Authors:  S N Cohen
Journal:  Gene       Date:  1993-12-15       Impact factor: 3.688

9.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

10.  Repressor titration: a novel system for selection and stable maintenance of recombinant plasmids.

Authors:  S G Williams; R M Cranenburgh; A M Weiss; C J Wrighton; D J Sherratt; J A Hanak
Journal:  Nucleic Acids Res       Date:  1998-05-01       Impact factor: 16.971
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  10 in total

1.  Identification, characterization and field testing of Brassica napus mutants producing high-oleic oils.

Authors:  Shuangyi Bai; Steven Engelen; Peter Denolf; James G Wallis; Katherine Lynch; Jesse D Bengtsson; Michel Van Thournout; Boris Haesendonckx; John Browse
Journal:  Plant J       Date:  2019-01-28       Impact factor: 6.417

2.  A novel automethylation reaction in the Aspergillus nidulans LaeA protein generates S-methylmethionine.

Authors:  Alexander N Patananan; Jonathan M Palmer; Graeme S Garvey; Nancy P Keller; Steven G Clarke
Journal:  J Biol Chem       Date:  2013-03-26       Impact factor: 5.157

3.  bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans.

Authors:  Wen-Bing Yin; Aaron W Reinke; Melinda Szilágyi; Tamás Emri; Yi-Ming Chiang; Amy E Keating; István Pócsi; Clay C C Wang; Nancy P Keller
Journal:  Microbiology       Date:  2012-11-15       Impact factor: 2.777

4.  VeA and MvlA repression of the cryptic orsellinic acid gene cluster in Aspergillus nidulans involves histone 3 acetylation.

Authors:  Jin Woo Bok; Alexandra A Soukup; Elizabeth Chadwick; Yi-Ming Chiang; Clay C C Wang; Nancy P Keller
Journal:  Mol Microbiol       Date:  2013-08-01       Impact factor: 3.501

5.  Fungal artificial chromosomes for mining of the fungal secondary metabolome.

Authors:  Jin Woo Bok; Rosa Ye; Kenneth D Clevenger; David Mead; Megan Wagner; Amanda Krerowicz; Jessica C Albright; Anthony W Goering; Paul M Thomas; Neil L Kelleher; Nancy P Keller; Chengcang C Wu
Journal:  BMC Genomics       Date:  2015-04-29       Impact factor: 3.969

6.  Lipoxygenase Activity Accelerates Programmed Spore Germination in Aspergillus fumigatus.

Authors:  Gregory J Fischer; William Bacon; Jun Yang; Jonathan M Palmer; Taylor Dagenais; Bruce D Hammock; Nancy P Keller
Journal:  Front Microbiol       Date:  2017-05-09       Impact factor: 5.640

7.  APOBEC3-mediated restriction of RNA virus replication.

Authors:  Aleksandra Milewska; Eveline Kindler; Philip Vkovski; Slawomir Zeglen; Marek Ochman; Volker Thiel; Zenon Rajfur; Krzysztof Pyrc
Journal:  Sci Rep       Date:  2018-04-13       Impact factor: 4.379

8.  Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers.

Authors:  Marijn de Boer; Giorgos Gouridis; Ruslan Vietrov; Stephanie L Begg; Gea K Schuurman-Wolters; Florence Husada; Nikolaos Eleftheriadis; Bert Poolman; Christopher A McDevitt; Thorben Cordes
Journal:  Elife       Date:  2019-03-22       Impact factor: 8.140

9.  Single-Molecule Observation of Ligand Binding and Conformational Changes in FeuA.

Authors:  Marijn de Boer; Giorgos Gouridis; Yusran Abdillah Muthahari; Thorben Cordes
Journal:  Biophys J       Date:  2019-08-12       Impact factor: 4.033

10.  The bZIP protein MeaB mediates virulence attributes in Aspergillus flavus.

Authors:  Saori Amaike; Katharyn J Affeldt; Wen-Bing Yin; Stephen Franke; Anjali Choithani; Nancy P Keller
Journal:  PLoS One       Date:  2013-09-09       Impact factor: 3.240
  10 in total

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