PROBLEM: Local adaptive cervical regulatory T cells (Tregs) are the most likely direct suppressors of the immune eradication of cervical intraepithelial lesion (CIN). PD-1 expression on T cells induces Tregs. No studies have quantitatively analyzed the Tregs and PD-1+ cells residing in CIN lesions. METHOD OF STUDY: Cervical lymphocytes were collected using cytobrushes from CIN patients and analyzed by FACS analysis. Comparisons were made between populations of cervical Tregs and PD-1+ CD4+ T cells in CIN regressors and non-regressors. RESULTS: A median of 11% of cervical CD4+ T cells were Tregs, while a median of 30% were PD-1+ cells. The proportions of cervical CD4+ T cells that were Tregs and/or PD-1+ cells were significantly lower in CIN regressors when compared with non-regressors. CONCLUSIONS: The prevalence of cervical tolerogenic T cells correlates inversely with spontaneous regression of CIN. Cervical Tregs may play an important role in HPV-related neoplastic immunoevasion.
PROBLEM: Local adaptive cervical regulatory T cells (Tregs) are the most likely direct suppressors of the immune eradication of cervical intraepithelial lesion (CIN). PD-1 expression on T cells induces Tregs. No studies have quantitatively analyzed the Tregs and PD-1+ cells residing in CIN lesions. METHOD OF STUDY: Cervical lymphocytes were collected using cytobrushes from CINpatients and analyzed by FACS analysis. Comparisons were made between populations of cervical Tregs and PD-1+ CD4+ T cells in CIN regressors and non-regressors. RESULTS: A median of 11% of cervical CD4+ T cells were Tregs, while a median of 30% were PD-1+ cells. The proportions of cervical CD4+ T cells that were Tregs and/or PD-1+ cells were significantly lower in CIN regressors when compared with non-regressors. CONCLUSIONS: The prevalence of cervical tolerogenic T cells correlates inversely with spontaneous regression of CIN. Cervical Tregs may play an important role in HPV-related neoplastic immunoevasion.
Authors: Linda Hertlein; Thomas Kolben; Aurelia Vattai; Nadine Kremer; Sarah Meister; Susanne Beyer; Lucia Keilmann; Anna Hester; Mina Temelkov; Helene Heidegger; Elisa Schmoeckel; Mirjana Kessler; Sven Mahner; Udo Jeschke Journal: J Cancer Res Clin Oncol Date: 2021-11-05 Impact factor: 4.553
Authors: Andrew W Woodham; Lisa Yan; Joseph G Skeate; Daniel van der Veen; Heike H Brand; Michael K Wong; Diane M Da Silva; W Martin Kast Journal: Papillomavirus Res Date: 2016-12
Authors: Krystle A Lang Kuhs; Shih-Wen Lin; Xing Hua; Mark Schiffman; Robert D Burk; Ana Cecilia Rodriguez; Rolando Herrero; Christian C Abnet; Neal D Freedman; Ligia A Pinto; David Hamm; Harlan Robins; Allan Hildesheim; Jianxin Shi; Mahboobeh Safaeian Journal: PLoS One Date: 2018-01-31 Impact factor: 3.240