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Literature DB >> 23055266

In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.

Erkin Kuru¹, H Velocity Hughes, Pamela J Brown, Edward Hall, Srinivas Tekkam, Felipe Cava, Miguel A de Pedro, Yves V Brun, Michael S VanNieuwenhze.

Abstract

Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.

Entities: CellLine Chemical Disease Species

Mesh：

Substances：

Year: 2012 PMID： 23055266 PMCID： PMC3589519 DOI： 10.1002/anie.201206749

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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