Stefany D Primeaux1, H Douglas Braymer, George A Bray. 1. Joint Diabetes, Endocrinology and Metabolism Program, Louisiana State University System, Louisiana State University Health Science Center-New Orleans, New Orleans, LA 70112, USA. sprime@lsuhsc.edu
Abstract
BACKGROUND: The gastrointestinal tract is important in the regulation of food intake, nutrient sensing and nutrient absorption. Obesity-prone Osborne-Mendel (OM) rats are less sensitive to the satiating effects of a duodenal infusion of fatty acids than obesity-resistant S5B/Pl (S5B) rats, suggesting that the gastrointestinal tract differentially senses the presence of fat in these two strains. A microarray analysis was conducted to identify genes that were differentially expressed in the duodenal enterocytes of OM and S5B rats. AIMS: The present experiment evaluated the expression of olfactory receptors in the duodenal enterocytes of OM and S5B rats. It was hypothesized that olfactory receptors present in the duodenum of OM and S5B rats would be differentially regulated by the intake of a high fat diet. METHODS: The mRNA levels of four olfactory receptors (Olr1744, Olr50, Olr124, Olr1507) were assessed from the duodenal enterocytes of OM and S5B rats consuming a high fat diet for 14 days. RESULTS: The duodenal mRNA levels of Olr1744, Olr124 and Olr1507 were significantly elevated in OM rats fed the high fat diet, but not S5B rats. No differences in the expression of Olr50 receptor mRNA were detected. CONCLUSIONS: These data suggest that several olfactory receptors present in the duodenum are selectively regulated by high fat diet intake in obesity-prone OM rats. Therefore, these receptors may play a role in the sensing and regulation of dietary fat, and may be important for the individual susceptibility to obesity in these two strains.
BACKGROUND: The gastrointestinal tract is important in the regulation of food intake, nutrient sensing and nutrient absorption. Obesity-prone Osborne-Mendel (OM) rats are less sensitive to the satiating effects of a duodenal infusion of fatty acids than obesity-resistant S5B/Pl (S5B) rats, suggesting that the gastrointestinal tract differentially senses the presence of fat in these two strains. A microarray analysis was conducted to identify genes that were differentially expressed in the duodenal enterocytes of OM and S5B rats. AIMS: The present experiment evaluated the expression of olfactory receptors in the duodenal enterocytes of OM and S5B rats. It was hypothesized that olfactory receptors present in the duodenum of OM and S5B rats would be differentially regulated by the intake of a high fat diet. METHODS: The mRNA levels of four olfactory receptors (Olr1744, Olr50, Olr124, Olr1507) were assessed from the duodenal enterocytes of OM and S5B rats consuming a high fat diet for 14 days. RESULTS: The duodenal mRNA levels of Olr1744, Olr124 and Olr1507 were significantly elevated in OM rats fed the high fat diet, but not S5B rats. No differences in the expression of Olr50 receptor mRNA were detected. CONCLUSIONS: These data suggest that several olfactory receptors present in the duodenum are selectively regulated by high fat diet intake in obesity-prone OM rats. Therefore, these receptors may play a role in the sensing and regulation of dietary fat, and may be important for the individual susceptibility to obesity in these two strains.
Authors: Michal Pravenec; Laura M Saba; Václav Zídek; Vladimír Landa; Petr Mlejnek; Jan Šilhavý; Miroslava Šimáková; Hynek Strnad; Jaroslava Trnovská; Vojtěch Škop; Martina Hüttl; Irena Marková; Olena Oliyarnyk; Hana Malínská; Ludmila Kazdová; Harry Smith; Boris Tabakoff Journal: Physiol Genomics Date: 2017-11-10 Impact factor: 3.107
Authors: R G Vink; N J Roumans; P Fazelzadeh; S H K Tareen; M V Boekschoten; M A van Baak; E C Mariman Journal: Int J Obes (Lond) Date: 2016-11-14 Impact factor: 5.095
Authors: Jonquil M Poret; Claire Battle; Alan J Mouton; Darryl A Gaudet; Flavia Souza-Smith; Jason D Gardner; H Douglas Braymer; Lisa Harrison-Bernard; Stefany D Primeaux Journal: Life Sci Date: 2019-03-11 Impact factor: 5.037