Hsiao-Chuan Lin1, Hsiang-Yu Lin2, Bai-Hong Su3, Mao-Wang Ho4, Cheng-Mao Ho5, Ching-Yi Lee6, Ming-Hsia Lin2, Hsin-Yang Hsieh2, Hung-Chih Lin2, Tsai-Chung Li7, Kao-Pin Hwang2, Jang-Jih Lu8. 1. Department of Public Health, China Medical University, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan; Department of Pediatrics, China Medical University Hospital, Taichung, Taiwan. 2. Department of Pediatrics, China Medical University Hospital, Taichung, Taiwan. 3. School of Medicine, China Medical University, Taichung, Taiwan; Department of Pediatrics, China Medical University Hospital, Taichung, Taiwan. 4. Section of Infectious Diseases, Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan. 5. Section of Infectious Diseases, Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan; Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan. 6. Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan. 7. Institute of Health Care Administration, College of Public Health, China Medical University and Hospital, Taichung, Taiwan; Graduate Institute of Biostatistics and Chinese Medicine Science, China Medical University and Hospital, Taichung, Taiwan; Biostatistics Center, China Medical University and Hospital, Taichung, Taiwan. 8. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan; Department of Laboratory Medicine, Chang-Gung Memorial Hospital, Linkou, Taoyuan, Taiwan. Electronic address: janglu45@gmail.com.
Abstract
BACKGROUND: Fungemia in preterm infants is associated with high mortality and morbidity. This study reports an outbreak of unusual fungemia in a tertiary neonatal intensive care unit (NICU). METHODS: Ten Candida pelliculosa bloodstream isolates were identified from six infants hospitalized in the NICU from February to March 2009. Environmental study was performed, and genetic relatedness among the 10 clinical isolates of C pelliculosa and six control C pelliculosa strains was characterized by randomly amplified polymorphic DNA assay. In vitro susceptibility of isolates to six antifungal agents was analyzed by broth microdilution method. Amphotericin B was given to infected infants and prophylactic fluconazole was prescribed to the other noninfected extremely low birth weight infants during the outbreak. RESULTS: Thrombocytopenia (platelet counts <100×10(9)/L) was the early laboratory finding in four infants. One of six patients died, making overall mortality 17%. Fluconazole, voriconazole, amphotericin B, and micafungin provided good antifungal activity. Cultures from the environment and hands of caregivers were all negative. Molecular studies indicated the outbreak as caused by a single strain. The outbreak was controlled by strict hand washing, cohort infected patients, confined physicians and nurses to take care of patients, prophylactic fluconazole to uninfected neonates, and proper management of human milk. CONCLUSION: The study demonstrated the clinical importance of emerged non-albicans Candida species in NICU. For unusual pathogen isolated from immunocompromised hosts, more attention should be paid to monitor the possibility of an outbreak.
BACKGROUND:Fungemia in preterm infants is associated with high mortality and morbidity. This study reports an outbreak of unusual fungemia in a tertiary neonatal intensive care unit (NICU). METHODS: Ten Candida pelliculosa bloodstream isolates were identified from six infants hospitalized in the NICU from February to March 2009. Environmental study was performed, and genetic relatedness among the 10 clinical isolates of C pelliculosa and six control C pelliculosa strains was characterized by randomly amplified polymorphic DNA assay. In vitro susceptibility of isolates to six antifungal agents was analyzed by broth microdilution method. Amphotericin B was given to infected infants and prophylactic fluconazole was prescribed to the other noninfected extremely low birth weight infants during the outbreak. RESULTS:Thrombocytopenia (platelet counts <100×10(9)/L) was the early laboratory finding in four infants. One of six patients died, making overall mortality 17%. Fluconazole, voriconazole, amphotericin B, and micafungin provided good antifungal activity. Cultures from the environment and hands of caregivers were all negative. Molecular studies indicated the outbreak as caused by a single strain. The outbreak was controlled by strict hand washing, cohort infectedpatients, confined physicians and nurses to take care of patients, prophylactic fluconazole to uninfected neonates, and proper management of human milk. CONCLUSION: The study demonstrated the clinical importance of emerged non-albicans Candida species in NICU. For unusual pathogen isolated from immunocompromised hosts, more attention should be paid to monitor the possibility of an outbreak.