AIM: The aim of this study was to evaluate the effect of the surface functionalization of model nanoparticles on their mobility in bacterial biofilms and cystic fibrosis sputum. MATERIALS & METHODS: With single-particle tracking microscopy, the mobility of 0.1- and 0.2-µm fluorescent polyethylene glycol (PEG) modified, carboxylate- and N,N-dimethylethylenediamine-modified polystyrene nanospheres were evaluated in fresh cystic fibrosis sputum, as well as Burkholderia multivorans and Pseudomonas aeruginosa biofilms. RESULTS: PEGylation increased the mobility of the particles in sputum and biofilms, while the charged nanospheres were strongly immobilized. However, the transport of the PEGylated nanoparticles was lower in sputum compared with biofilms. Furthermore, the particle transport showed heterogeneity in samples originating from different patients. CONCLUSION: This study's data suggest that for future nanocarrier design it will be essential to combine PEGylation with a targeting moiety to ensure sufficient mobility in mucus and a better accumulation of the nanoparticles in the biofilm.
AIM: The aim of this study was to evaluate the effect of the surface functionalization of model nanoparticles on their mobility in bacterial biofilms and cystic fibrosis sputum. MATERIALS & METHODS: With single-particle tracking microscopy, the mobility of 0.1- and 0.2-µm fluorescent polyethylene glycol (PEG) modified, carboxylate- and N,N-dimethylethylenediamine-modified polystyrene nanospheres were evaluated in fresh cystic fibrosis sputum, as well as Burkholderia multivorans and Pseudomonas aeruginosa biofilms. RESULTS: PEGylation increased the mobility of the particles in sputum and biofilms, while the charged nanospheres were strongly immobilized. However, the transport of the PEGylated nanoparticles was lower in sputum compared with biofilms. Furthermore, the particle transport showed heterogeneity in samples originating from different patients. CONCLUSION: This study's data suggest that for future nanocarrier design it will be essential to combine PEGylation with a targeting moiety to ensure sufficient mobility in mucus and a better accumulation of the nanoparticles in the biofilm.
Authors: Katelyn P Reighard; Camille Ehre; Zachary L Rushton; Mona Jasmine R Ahonen; David B Hill; Mark H Schoenfisch Journal: ACS Biomater Sci Eng Date: 2017-05-15
Authors: Benjamin S Schuster; Laura M Ensign; Daniel B Allan; Jung Soo Suk; Justin Hanes Journal: Adv Drug Deliv Rev Date: 2015-04-07 Impact factor: 15.470
Authors: Benjamin S Schuster; Anthony J Kim; Joshua C Kays; Mia M Kanzawa; William B Guggino; Michael P Boyle; Steven M Rowe; Nicholas Muzyczka; Jung Soo Suk; Justin Hanes Journal: Mol Ther Date: 2014-05-29 Impact factor: 11.454
Authors: Young H Lim; Kristin M Tiemann; David A Hunstad; Mahmoud Elsabahy; Karen L Wooley Journal: Wiley Interdiscip Rev Nanomed Nanobiotechnol Date: 2016-03-25