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Literature DB >> 23027009

Measuring the elastic properties of living cells with atomic force microscopy indentation.

Abstract

Atomic force microscopy (AFM) is a powerful and versatile tool for probing the mechanical properties of biological samples. This chapter describes the procedures for using AFM indentation to measure the elastic moduli of living cells. We include step-by-step instructions for cantilever calibration and data acquisition using a combined AFM/optical microscope system, as well as a detailed protocol for data analysis. Our protocol is written specifically for the BioScope™ Catalyst™ AFM system (Bruker AXS Inc.); however, most of the general concepts can be readily translated to other commercial systems.

Mesh：

Year: 2013 PMID： 23027009 DOI： 10.1007/978-1-62703-056-4_15

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

17 in total

1. Macrophage adhesion on fibronectin evokes an increase in the elastic property of the cell membrane and cytoskeleton: an atomic force microscopy study.

Authors: Samuel T Souza; Laís C Agra; Cássio E A Santos; Emiliano Barreto; Jandir M Hickmann; Eduardo J S Fonseca
Journal: Eur Biophys J Date: 2014-10-19 Impact factor: 1.733

2. Two-bubble acoustic tweezing cytometry for biomechanical probing and stimulation of cells.

Authors: Di Chen; Yubing Sun; Madhu S R Gudur; Yi-Sing Hsiao; Ziqi Wu; Jianping Fu; Cheri X Deng
Journal: Biophys J Date: 2015-01-06 Impact factor: 4.033

3. Longer collagen fibers trigger multicellular streaming on soft substrates via enhanced forces and cell-cell cooperation.

Authors: Bapi Sarker; Amrit Bagchi; Christopher Walter; José Almeida; Amit Pathak
Journal: J Cell Sci Date: 2019-09-26 Impact factor: 5.285

4. Past matrix stiffness primes epithelial cells and regulates their future collective migration through a mechanical memory.

Authors: Samila Nasrollahi; Christopher Walter; Andrew J Loza; Gregory V Schimizzi; Gregory D Longmore; Amit Pathak
Journal: Biomaterials Date: 2017-09-08 Impact factor: 12.479

5. Stiffness of pancreatic cancer cells is associated with increased invasive potential.

Authors: Angelyn V Nguyen; Kendra D Nyberg; Michael B Scott; Alia M Welsh; Andrew H Nguyen; Nanping Wu; Sophia V Hohlbauch; Nicholas A Geisse; Ewan A Gibb; A Gordon Robertson; Timothy R Donahue; Amy C Rowat
Journal: Integr Biol (Camb) Date: 2016-12-05 Impact factor: 2.192

Measuring the elastic properties of living cells with atomic force microscopy indentation.

1. Macrophage adhesion on fibronectin evokes an increase in the elastic property of the cell membrane and cytoskeleton: an atomic force microscopy study.

2. Two-bubble acoustic tweezing cytometry for biomechanical probing and stimulation of cells.

3. Longer collagen fibers trigger multicellular streaming on soft substrates via enhanced forces and cell-cell cooperation.

4. Past matrix stiffness primes epithelial cells and regulates their future collective migration through a mechanical memory.

5. Stiffness of pancreatic cancer cells is associated with increased invasive potential.

6. Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell-ECM Interactions.

7. Differential role of nonmuscle myosin II isoforms during blebbing of MCF-7 cells.

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10. MMP proteolytic activity regulates cancer invasiveness by modulating integrins.