Maghsod Shaaker1, Ali Rahimipour2, Mohammad Nouri3, Korosh Khanaki3, Masoud Darabi3,4, Laya Farzadi5, Vahideh Shahnazi5, Amir Mehdizadeh3. 1. Laboratory of Chromatography, Dept. of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. a.mehdizadeh@hotmail.com. 2. Faculty of Para Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Laboratory of Chromatography, Dept. of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 4. 4Dept. of Medical Biotechnology, Faculty of Advanced Biomedical Sciences, zzm321990Tabriz University of Medical Sciences, Tabriz, Iran. 5. Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
BACKGROUND: Fatty acids are known to be critically important in multiple biological functions. Phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women's fertility and the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of fatty acid composition of follicular fluid phospholipids on women undergoing assisted reproductive techniques. METHODS: Follicular fluid samples were obtained from 100 patients, referred to Tabriz Alzahra Hospital. Seventy-nine subjects underwent in vitro fertilization (IVF) and the remaining 21 underwent intracytoplasmic sperm injection (ICSI). Total lipid of follicular fluid was extracted and fatty acids were analyzed by gas-liquid chromatography. RESULTS: Saturated fatty acids (SFA, P = 0.002) and the ratio of SFA to polyunsaturated fatty acids (P = 0.001) were correlated negatively with a number of mature oocytes after age adjustment. Linoleic acid (P = 0.006) was positively correlated, while the level of arachidonic acid was negatively correlated with fertility percentage after adjustment for body mass index, sperm count, sperm motility. CONCLUSION: Since phospholipids are one of the major components of lipid metabolism, the results of this study highlight the importance of this component in follicular fluid lipid metabolism. Consequently, it is proposed as an index in determination of the rate of success in assisted reproductive techniques such as IVF/ICSI.
BACKGROUND:Fatty acids are known to be critically important in multiple biological functions. Phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women's fertility and the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of fatty acid composition of follicular fluid phospholipids on women undergoing assisted reproductive techniques. METHODS: Follicular fluid samples were obtained from 100 patients, referred to Tabriz Alzahra Hospital. Seventy-nine subjects underwent in vitro fertilization (IVF) and the remaining 21 underwent intracytoplasmic sperm injection (ICSI). Total lipid of follicular fluid was extracted and fatty acids were analyzed by gas-liquid chromatography. RESULTS:Saturated fatty acids (SFA, P = 0.002) and the ratio of SFA to polyunsaturated fatty acids (P = 0.001) were correlated negatively with a number of mature oocytes after age adjustment. Linoleic acid (P = 0.006) was positively correlated, while the level of arachidonic acid was negatively correlated with fertility percentage after adjustment for body mass index, sperm count, sperm motility. CONCLUSION: Since phospholipids are one of the major components of lipid metabolism, the results of this study highlight the importance of this component in follicular fluid lipid metabolism. Consequently, it is proposed as an index in determination of the rate of success in assisted reproductive techniques such as IVF/ICSI.
Authors: T R Bilby; J Block; B C do Amaral; O Sa Filho; F T Silvestre; P J Hansen; C R Staples; W W Thatcher Journal: J Dairy Sci Date: 2006-10 Impact factor: 4.034
Authors: Hilde Aardema; Peter L A M Vos; Francesca Lolicato; Bernard A J Roelen; Hiemke M Knijn; Arie B Vaandrager; J Bernd Helms; Bart M Gadella Journal: Biol Reprod Date: 2011-02-10 Impact factor: 4.285
Authors: Ali A Fouladi-Nashta; Karen E Wonnacott; Carlos G Gutierrez; Jin G Gong; Kevin D Sinclair; Philip C Garnsworthy; Robert Webb Journal: Reproduction Date: 2009-07-24 Impact factor: 3.906
Authors: Natalie M Hohos; Kirstin J Cho; Delaney C Swindle; Amanda A Allshouse; Michael C Rudolph; Malgorzata E Skaznik-Wikiel Journal: Endocrinology Date: 2018-12-01 Impact factor: 4.736
Authors: Y J Liu; B Vlaeminck; K Tilleman; P Y Chouinard; R Gervais; P De Sutter; V Fievez Journal: J Assist Reprod Genet Date: 2020-01-18 Impact factor: 3.412
Authors: Natalie M Hohos; Emily M Elliott; Kirstin J Cho; Ivy S Lin; Michael C Rudolph; Malgorzata E Skaznik-Wikiel Journal: Mol Cell Endocrinol Date: 2019-10-16 Impact factor: 4.102
Authors: Sara D M Valckx; Maria Arias-Alvarez; Ingrid De Pauw; Veerle Fievez; Bruno Vlaeminck; Erik Fransen; Peter E J Bols; Jo L M R Leroy Journal: Reprod Biol Endocrinol Date: 2014-02-05 Impact factor: 5.211
Authors: Lisa J Moran; Laura Spencer; Darryl L Russell; Mary Louise Hull; Sarah A Robertson; Tamara J Varcoe; Michael J Davies; Hannah M Brown; Raymond J Rodgers Journal: Nutrients Date: 2016-01-13 Impact factor: 5.717