Literature DB >> 23019116

Phasor-FLIM analysis of NADH distribution and localization in the nucleus of live progenitor myoblast cells.

Belinda K Wright1, Laura M Andrews, Mark R Jones, Chiara Stringari, Michelle A Digman, Enrico Gratton.   

Abstract

Analysis of the cellular distributions of coenzymes including NADH may aid in understanding a cells metabolic status. We altered serum concentration (0, 2, and 10%) to induce living myoblast cells to undergo the early stages of differentiation. Through microscopy and phasor-FLIM, we spatially mapped and identified variations in the distribution of free and bound NADH. Undifferentiated cells displayed abundant free NADH within the nucleus along with specific regions of more bound NADH. Complete serum starvation dramatically increased the fraction of bound NADH in the nucleus, indicating heightened requirement for transcriptional processes. In comparison, cells exposed to 2% serum exhibited intermediate free nuclear NADH fraction. Overall our results suggest an order of events in which a cell metabolic status alters significantly during the early stages of serum induced differentiation.
Copyright © 2012 Wiley Periodicals, Inc.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 23019116      PMCID： PMC3635844          DOI： 10.1002/jemt.22121

Source DB:  PubMed          Journal:  Microsc Res Tech        ISSN： 1059-910X            Impact factor:   2.769


  16 in total

Review 1.  Structures and proton-pumping strategies of mitochondrial respiratory enzymes.

Authors:  B E Schultz; S I Chan
Journal:  Annu Rev Biophys Biomol Struct       Date:  2001

2.  CtBP represses p300-mediated transcriptional activation by direct association with its bromodomain.

Authors:  Jae-Hwan Kim; Eun-Jung Cho; Seong-Tae Kim; Hong-Duk Youn
Journal:  Nat Struct Mol Biol       Date:  2005-04-17       Impact factor: 15.369

3.  Hitting transcription in all the right places.

Authors:  Erwan Lejeune; Andreas G Ladurner
Journal:  Nat Struct Mol Biol       Date:  2005-05       Impact factor: 15.369

Review 4.  Fluorescence lifetime imaging microscopy (FLIM).

Authors:  Erik B van Munster; Theodorus W J Gadella
Journal:  Adv Biochem Eng Biotechnol       Date:  2005       Impact factor: 2.635

5.  NADH distribution in live progenitor stem cells by phasor-fluorescence lifetime image microscopy.

Authors:  Belinda K Wright; Laura M Andrews; Julie Markham; Mark R Jones; Chiara Stringari; Michelle A Digman; Enrico Gratton
Journal:  Biophys J       Date:  2012-07-03       Impact factor: 4.033

6.  Respiratory activity of isolated mammalian nuclei. 3. The reduced nicotinamide adenine dinucleotide oxidase of rat liver nuclei and nucleoli.

Authors:  W D Currie; N M Davidian; W B Elliott; N F Rodman; R Penniall
Journal:  Arch Biochem Biophys       Date:  1966-01       Impact factor: 4.013

Review 7.  Multifrequency phase and modulation fluorometry.

Authors:  E Gratton; D M Jameson; R D Hall
Journal:  Annu Rev Biophys Bioeng       Date:  1984

8.  Resolution of mixtures of fluorophores using variable-frequency phase and modulation data.

Authors:  E Gratton; M Limkeman; J R Lakowicz; B P Maliwal; H Cherek; G Laczko
Journal:  Biophys J       Date:  1984-10       Impact factor: 4.033

Review 9.  Nicotinamide adenine dinucleotide, a metabolic regulator of transcription, longevity and disease.

Authors:  Su-Ju Lin; Leonard Guarente
Journal:  Curr Opin Cell Biol       Date:  2003-04       Impact factor: 8.382

10.  Differential binding of NAD+ and NADH allows the transcriptional corepressor carboxyl-terminal binding protein to serve as a metabolic sensor.

Authors:  Clark C Fjeld; William T Birdsong; Richard H Goodman
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-18       Impact factor: 11.205
View more
  19 in total

1.  Fluorescence lifetime microscopy of NADH distinguishes alterations in cerebral metabolism in vivo.

Authors:  Mohammad A Yaseen; Jason Sutin; Weicheng Wu; Buyin Fu; Hana Uhlirova; Anna Devor; David A Boas; Sava Sakadžić
Journal:  Biomed Opt Express       Date:  2017-04-03       Impact factor: 3.732

2.  Measurements of absolute concentrations of NADH in cells using the phasor FLIM method.

Authors:  Ning Ma; Michelle A Digman; Leonel Malacrida; Enrico Gratton
Journal:  Biomed Opt Express       Date:  2016-06-01       Impact factor: 3.732

3.  Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy.

Authors:  Alba Alfonso-García; Tim D Smith; Rupsa Datta; Thuy U Luu; Enrico Gratton; Eric O Potma; Wendy F Liu
Journal:  J Biomed Opt       Date:  2016-04-30       Impact factor: 3.170

4.  Spatial dynamics of SIRT1 and the subnuclear distribution of NADH species.

Authors:  Lorena Aguilar-Arnal; Suman Ranjit; Chiara Stringari; Ricardo Orozco-Solis; Enrico Gratton; Paolo Sassone-Corsi
Journal:  Proc Natl Acad Sci U S A       Date:  2016-10-24       Impact factor: 11.205

5.  Slide-free virtual histochemistry (Part II): detection of field cancerization.

Authors:  Sixian You; Yi Sun; Eric J Chaney; Youbo Zhao; Jianxin Chen; Stephen A Boppart; Haohua Tu
Journal:  Biomed Opt Express       Date:  2018-10-05       Impact factor: 3.732

6.  Characterizing fibrosis in UUO mice model using multiparametric analysis of phasor distribution from FLIM images.

Authors:  Suman Ranjit; Alexander Dvornikov; Moshe Levi; Seth Furgeson; Enrico Gratton
Journal:  Biomed Opt Express       Date:  2016-08-17       Impact factor: 3.732

7.  μMAPPS: a novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure.

Authors:  F Radaelli; L D'Alfonso; M Collini; F Mingozzi; L Marongiu; F Granucci; I Zanoni; G Chirico; L Sironi
Journal:  Sci Rep       Date:  2017-12-12       Impact factor: 4.379

Review 8.  Oncostatic-Cytoprotective Effect of Melatonin and Other Bioactive Molecules: A Common Target in Mitochondrial Respiration.

Authors:  Nicola Pacini; Fabio Borziani
Journal:  Int J Mol Sci       Date:  2016-03-07       Impact factor: 5.923

9.  Dextran-Catechin: An anticancer chemically-modified natural compound targeting copper that attenuates neuroblastoma growth.

Authors:  Orazio Vittorio; Miriam Brandl; Giuseppe Cirillo; Kathleen Kimpton; Elizabeth Hinde; Katharina Gaus; Eugene Yee; Naresh Kumar; Hien Duong; Claudia Fleming; Michelle Haber; Murray Norris; Cyrille Boyer; Maria Kavallaris
Journal:  Oncotarget       Date:  2016-07-26

Review 10.  Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging.

Authors:  Andrew W Browne; Cosimo Arnesano; Narine Harutyunyan; Thien Khuu; Juan Carlos Martinez; Harvey A Pollack; David S Koos; Thomas C Lee; Scott E Fraser; Rex A Moats; Jennifer G Aparicio; David Cobrinik
Journal:  Invest Ophthalmol Vis Sci       Date:  2017-07-01       Impact factor: 4.799
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.