Literature DB >> 23015491

Genetic manipulation of a naturally competent bacterium, Helicobacter pylori.

Jennifer M Noto1, Richard M Peek.   

Abstract

Genetic manipulation of Helicobacter pylori facilitates characterization and functional analysis of individual H. pylori genes. This chapter discusses the methods involved in H. pylori chromosomal DNA isolation, mutagenesis of individual genes, and natural transformation.

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Year:  2012        PMID： 23015491      PMCID： PMC3544407          DOI： 10.1007/978-1-62703-005-2_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  The complete genome sequence of the gastric pathogen Helicobacter pylori.

Authors:  J F Tomb; O White; A R Kerlavage; R A Clayton; G G Sutton; R D Fleischmann; K A Ketchum; H P Klenk; S Gill; B A Dougherty; K Nelson; J Quackenbush; L Zhou; E F Kirkness; S Peterson; B Loftus; D Richardson; R Dodson; H G Khalak; A Glodek; K McKenney; L M Fitzegerald; N Lee; M D Adams; E K Hickey; D E Berg; J D Gocayne; T R Utterback; J D Peterson; J M Kelley; M D Cotton; J M Weidman; C Fujii; C Bowman; L Watthey; E Wallin; W S Hayes; M Borodovsky; P D Karp; H O Smith; C M Fraser; J C Venter
Journal:  Nature       Date:  1997-08-07       Impact factor: 49.962

2.  Preparation of genomic DNA from bacteria.

Authors:  K Wilson
Journal:  Curr Protoc Mol Biol       Date:  2001-11

3.  Directed mutagenesis using the polymerase chain reaction.

Authors:  B Cormack
Journal:  Curr Protoc Mol Biol       Date:  2001-05

4.  Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.

Authors:  R M Horton; Z L Cai; S N Ho; L R Pease
Journal:  Biotechniques       Date:  1990-05       Impact factor: 1.993

5.  Comparative genomics of Helicobacter pylori: analysis of the outer membrane protein families.

Authors:  R A Alm; J Bina; B M Andrews; P Doig; R E Hancock; T J Trust
Journal:  Infect Immun       Date:  2000-07       Impact factor: 3.441

6.  A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains.

Authors:  N Salama; K Guillemin; T K McDaniel; G Sherlock; L Tompkins; S Falkow
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-19       Impact factor: 11.205

7.  Population genetic analysis of Helicobacter pylori by multilocus enzyme electrophoresis: extensive allelic diversity and recombinational population structure.

Authors:  M F Go; V Kapur; D Y Graham; J M Musser
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490
  7 in total
  2 in total

1.  Control of gene expression in Helicobacter pylori using the Tet repressor.

Authors:  Mark S McClain; Stacy S Duncan; Jennifer A Gaddy; Timothy L Cover
Journal:  J Microbiol Methods       Date:  2013-10-08       Impact factor: 2.363

2.  Phylogenomics of Colombian Helicobacter pylori isolates.

Authors:  Andrés Julián Gutiérrez-Escobar; Esperanza Trujillo; Orlando Acevedo; María Mercedes Bravo
Journal:  Gut Pathog       Date:  2017-09-11       Impact factor: 4.181
  2 in total

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