Literature DB >> 2300548

3H nuclear magnetic resonance study of anaerobic glycolysis in packed erythrocytes.

R D Newmark1, S Un, P G Williams, P J Carson, H Morimoto, M P Klein.   

Abstract

The utility and power of 3H NMR spectroscopy as a technique for monitoring biological systems in vivo is illustrated with glucose metabolism in erythrocytes. Use of C-1-tritiated glucose allowed us to monitor the disappearance of the alpha and beta tritons, with the production of lactate and 1H3HO (HTO), as well as some intermediates. Spin lattice relaxation times (T1) were measured to avoid T1 distortion of the spectral intensities. Detection of the formation of 1 mM tritiated water in the presence of 110 M H2O protons and deuterons allows the eventual fate of the label in the pentose shunt to be observed in vivo.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2300548      PMCID： PMC53309          DOI： 10.1073/pnas.87.2.583

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  Studies on erythrocyte glycolysis. 3. The effects of active cation transport, pH and inorganic phosphate concentration on erythrocyte glycolysis.

Authors:  S Minakami; H Yoshikawa
Journal:  J Biochem       Date:  1966-02       Impact factor: 3.387

2.  Interaction of purified yeast cytochrome P-450 and labelled benzo[a]pyrene studied by tritium nuclear-magnetic-resonance spectroscopy [proceedings].

Authors:  S Libor; J P Bloxsidge; J A Elvidge; J R Jones; L F Woods; A Wiseman
Journal:  Biochem Soc Trans       Date:  1980-02       Impact factor: 5.407

3.  Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport.

Authors:  K M Brindle; F F Brown; I D Campbell; C Grathwohl; P W Kuchel
Journal:  Biochem J       Date:  1979-04-15       Impact factor: 3.857

4.  Cellular applications of 31P and 13C nuclear magnetic resonance.

Authors:  R G Shulman; T R Brown; K Ugurbil; S Ogawa; S M Cohen; J A den Hollander
Journal:  Science       Date:  1979-07-13       Impact factor: 47.728

5.  Regulation of the human-erythrocyte hexose-monophosphate shunt under conditions of oxidative stress. A study using NMR spectroscopy, a kinetic isotope effect, a reconstituted system and computer simulation.

Authors:  D R Thorburn; P W Kuchel
Journal:  Eur J Biochem       Date:  1985-07-15

6.  High-resolution 13C nuclear magnetic resonance studies of glucose metabolism in Escherichia coli.

Authors:  K Ugurbil; T R Brown; J A den Hollander; P Glynn; R G Shulman
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

7.  13C nuclear magnetic resonance studies of anaerobic glycolysis in suspensions of yeast cells.

Authors:  J A den Hollander; T R Brown; K Ugurbil; R G Shulman
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

8.  Biosynthesis of porphyrins and corrins. 2. Isolation, purification, and NMR investigations of the porphobilinogen-deaminase covalent complex.

Authors:  J N Evans; G Burton; P E Fagerness; N E Mackenzie; A I Scott
Journal:  Biochemistry       Date:  1986-02-25       Impact factor: 3.162

9.  A multinuclear NMR study of 2,3-bisphosphoglycerate metabolism in the human erythrocyte.

Authors:  S T Oxley; R Porteous; K M Brindle; J Boyd; I D Campbell
Journal:  Biochim Biophys Acta       Date:  1984-09-14
  9 in total
  4 in total

1.  Allosteric and catalytic binding of S-adenosylmethionine to Escherichia coli DNA adenine methyltransferase monitored by 3H NMR.

Authors:  A Bergerat; W Guschlbauer; G V Fazakerley
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-01       Impact factor: 11.205

2.  Tritium NMR studies of the human carbonic anhydrase I-benzenesulfonamide complex.

Authors:  A S Culf; J T Gerig; P G Williams
Journal:  J Biomol NMR       Date:  1997-10       Impact factor: 2.835

3.  [2-3H]ATP synthesis and 3H NMR spectroscopy of enzyme-nucleotide complexes: ADP and ADP.Vi bound to myosin subfragment 1.

Authors:  S Highsmith; M Kubinec; D K Jaiswal; H Morimoto; P G Williams; D E Wemmer
Journal:  J Biomol NMR       Date:  1993-05       Impact factor: 2.835

4.  Measurement of pentose phosphate-pathway activity in a single incubation with [1,6-13C2,6,6-2H2]glucose.

Authors:  B D Ross; P B Kingsley; O Ben-Yoseph
Journal:  Biochem J       Date:  1994-08-15       Impact factor: 3.857
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.