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Literature DB >> 486105

Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport.

K M Brindle, F F Brown, I D Campbell, C Grathwohl, P W Kuchel.

Abstract

A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte.

Entities: Chemical Species

Mesh：

Substances：
Lactates
Alanine

Year: 1979 PMID： 486105 PMCID： PMC1161016 DOI： 10.1042/bj1800037

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. NMR studies of pH-induced transport of carboxylic acids across phospholipid vesicle membranes.

Authors: J A Cramer; J H Prestegard
Journal: Biochem Biophys Res Commun Date: 1977-03-21 Impact factor: 3.575

2. MIGRATION OF AMINO ACIDS ACROSS THE MEMBRANE OF THE HUMAN ERYTHROCYTE.

Authors: C G WINTER; H N CHRISTENSEN
Journal: J Biol Chem Date: 1964-03 Impact factor: 5.157

3. Determination of the temperature and pH dependence of glucose transfer across the human erythrocyte membrane measured by glucose exit.

Authors: A K SEN; W F WIDDAS
Journal: J Physiol Date: 1962-03 Impact factor: 5.182

4. Human erythrocyte metabolism studies by 1H spin echo NMR.

Authors: F F Brown; I D Campbell; P W Kuchel; D C Rabenstein
Journal: FEBS Lett Date: 1977-10-01 Impact factor: 4.124

5. NMR kinetic studies of the ionophore X-537A-mediated transport of manganous ions across phospholipid bilayers.

Authors: H Degani
Journal: Biochim Biophys Acta Date: 1978-04-04

6. Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance.

Authors: G Navon; R Navon; R G Shulman; T Yamane
Journal: Proc Natl Acad Sci U S A Date: 1978-02 Impact factor: 11.205

7. Determination of intracellular pH by 31P magnetic resonance.

Authors: R B Moon; J H Richards
Journal: J Biol Chem Date: 1973-10-25 Impact factor: 5.157

8. Glucose release measurements from liposomes with an oxygen electrode.

Authors: R Hertz; Y Barenholz
Journal: Biochim Biophys Acta Date: 1973-11-30

9. Water diffusion permeability of erythrocytes using an NMR technique.

Authors: T Conlon; R Outhred
Journal: Biochim Biophys Acta Date: 1972-11-02

10. The temperature dependence of the transport of L-leucine in human erythrocytes.

Authors: D G Hoare
Journal: J Physiol Date: 1972-03 Impact factor: 5.182

26 in total

1. Use of spin echo T(2) BOLD in assessment of cerebral misery perfusion at 1.5 T.

Authors: M Kavec; O H Gröhn; M I Kettunen; M J Silvennoinen; M Penttonen; R A Kauppinen
Journal: MAGMA Date: 2001-03 Impact factor: 2.310

2. A biochemical and NMR spectroscopic study of hydrazine in the isolated rat hepatocyte.

Authors: S Ghatineh; W Morgan; N E Preece; J A Timbrell
Journal: Arch Toxicol Date: 1992 Impact factor: 5.153

3. Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy.

Authors: P C Van Zijl; C T Moonen; P Faustino; J Pekar; O Kaplan; J S Cohen
Journal: Proc Natl Acad Sci U S A Date: 1991-04-15 Impact factor: 11.205

4. Spectroscopic applications of magnetic resonance to biomedical problems.

Authors: M J Kushmerick
Journal: Cardiovasc Intervent Radiol Date: 1986 Impact factor: 2.740

5. Two-site exchange revisited: a new method for extracting exchange parameters in biological systems.

Authors: R V Mulkern; A R Bleier; I K Adzamli; R G Spencer; T Sandor; F A Jolesz
Journal: Biophys J Date: 1989-02 Impact factor: 4.033

6. Does signal-attenuation on high-field T2-weighted MRI of the brain reflect regional cerebral iron deposition? Observations on the relationship between regional cerebral water proton T2 values and iron levels.

Authors: D J Brooks; P Luthert; D Gadian; C D Marsden
Journal: J Neurol Neurosurg Psychiatry Date: 1989-01 Impact factor: 10.154