| Literature DB >> 22992920 |
Abstract
Annexin-V magnetic-activated cell sorting (MACS) is a new tool to optimize sperm selection in assisted reproduction. The technique is based on the binding of superparamagnetic Annexin-microbeads to externalized phosphatidylserine at the outer leaflet of the plasma membrane of sperm with activated apoptosis signaling or membrane damage. The combination of Annexin-V MACS and density gradient centrifugation was demonstrated to enhance clinical pregnancy rates in ICSI cycles. This chapter focuses on the practical details of Annexin-V MACS.Entities:
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Year: 2013 PMID： 22992920 DOI： 10.1007/978-1-62703-038-0_23
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745