Literature DB >> 22992909

Sperm DNA fragmentation analysis using the TUNEL assay.

Rakesh Sharma1, Jayson Masaki, Ashok Agarwal.   

Abstract

Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labeling or the TUNEL assay is an important technique in the assessment of DNA damage. Semen samples are routinely assessed microscopically to assess their fertilization ability. In addition to routine semen analysis, the use of the TUNEL assay can provide information on the level of DNA damage present within a sample. This chapter provides a practical walk-through guide aimed at directing a researcher or a clinical facility interested in setting up and using TUNEL and flow cytometry or fluorescence microscopy for sperm DNA analysis.

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Mesh：

Year:  2013        PMID： 22992909     DOI： 10.1007/978-1-62703-038-0_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  21 in total

1.  Luminal fluid of epididymis and vas deferens contributes to sperm chromatin fragmentation.

Authors:  Joanna E Gawecka; Segal Boaz; Kay Kasperson; Hieu Nguyen; Donald P Evenson; W Steven Ward
Journal:  Hum Reprod       Date:  2015-10-13       Impact factor: 6.918

2.  Case report: the use of annexin V coupled with magnetic activated cell sorting in cryopreserved spermatozoa from a male cancer survivor: healthy twin newborns after two previous ICSI failures.

Authors:  María Belén Herrero; Géraldine Delbes; Jin-Tae Chung; Weon-Young Son; Hananel Holzer; William Buckett; Peter Chan
Journal:  J Assist Reprod Genet       Date:  2013-09-21       Impact factor: 3.412

3.  Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay using bench top flow cytometer for evaluation of sperm DNA fragmentation in fertility laboratories: protocol, reference values, and quality control.

Authors:  Rakesh Sharma; Gulfam Ahmad; Sandro C Esteves; Ashok Agarwal
Journal:  J Assist Reprod Genet       Date:  2016-01-16       Impact factor: 3.412

Review 4.  Effects of aging on the male reproductive system.

Authors:  Sezgin Gunes; Gulgez Neslihan Taskurt Hekim; Mehmet Alper Arslan; Ramazan Asci
Journal:  J Assist Reprod Genet       Date:  2016-02-11       Impact factor: 3.412

5.  Nuclear DNA Fragmentation in Boar Spermatozoa: Measurement Methods and Reproductive Performance Implications.

Authors:  Raquel Ausejo; Juan Manuel Martínez; Noelia Mendoza; Alfonso Bolarin; M Teresa Tejedor; Maria Victoria Falceto
Journal:  Front Vet Sci       Date:  2022-06-30

6.  Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality.

Authors:  Kumiko Takeda; Kyoko Uchiyama; Masashi Kinukawa; Takahiro Tagami; Masahiro Kaneda; Shinya Watanabe
Journal:  J Reprod Dev       Date:  2015-01-27       Impact factor: 2.214

7.  Sperm DNA fragmentation, recurrent implantation failure and recurrent miscarriage.

Authors:  Carol Coughlan; Helen Clarke; Rachel Cutting; Jane Saxton; Sarah Waite; William Ledger; Tinchiu Li; Allan A Pacey
Journal:  Asian J Androl       Date:  2015 Jul-Aug       Impact factor: 3.285

8.  Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial.

Authors:  Helena Malvezzi; Rakesh Sharma; Ashok Agarwal; Adel M Abuzenadah; Muhammad Abu-Elmagd
Journal:  Reprod Biol Endocrinol       Date:  2014-12-02       Impact factor: 5.211

Review 9.  Sperm DNA damage and its role in IVF and ICSI.

Authors:  Phil Vu Bach; Peter N Schlegel
Journal:  Basic Clin Androl       Date:  2016-12-05

10.  Semen parameters in men with varicocele: DNA fragmentation, chromatin packaging, mitochondrial membrane potential, and apoptosis.

Authors:  Felipe Dieamant; Claudia G Petersen; Ana L Mauri; Vanessa Conmar; Mariana Mattila; Laura D Vagnini; Adriana Renzi; Bruna P Costa; Camila Zamara; João Batista A Oliveira; Ricardo L R Baruffi; José G Franco
Journal:  JBRA Assist Reprod       Date:  2017-12-01
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