Literature DB >> 22990790

In situ fluorescence visualization of transcription sites and genomic Loci in blood stages of Plasmodium falciparum.

Liliana Mancio-Silva1, Artur Scherf.   

Abstract

Fluorescence-based techniques have been used extensively in the malaria field to study the functional role of nuclear organization and gene positioning in blood stages of the human malaria parasite, Plasmodium falciparum. In this chapter, we present optimized protocols for bromouridine (BrUTP) incorporation into nascent RNA in live parasites and fluorescent in situ hybridization (FISH) in fixed parasites. Methodology to perform various combinations of the FISH assay, as well as a basic approach for quantitative analysis of nuclear position, is also described.

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Year:  2013        PMID： 22990790      PMCID： PMC7116677          DOI： 10.1007/978-1-62703-026-7_24

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  Frequent ectopic recombination of virulence factor genes in telomeric chromosome clusters of P. falciparum.

Authors:  L H Freitas-Junior; E Bottius; L A Pirrit; K W Deitsch; C Scheidig; F Guinet; U Nehrbass; T E Wellems; A Scherf
Journal:  Nature       Date:  2000-10-26       Impact factor: 49.962

2.  Gene regulation through nuclear organization.

Authors:  Tom Sexton; Heiko Schober; Peter Fraser; Susan M Gasser
Journal:  Nat Struct Mol Biol       Date:  2007-11-05       Impact factor: 15.369

3.  Mechanisms underlying mutually exclusive expression of virulence genes by malaria parasites.

Authors:  Ron Dzikowski; Felomena Li; Borko Amulic; Andrew Eisberg; Matthias Frank; Suchit Patel; Thomas E Wellems; Kirk W Deitsch
Journal:  EMBO Rep       Date:  2007-08-31       Impact factor: 8.807

Review 4.  Transcription factories: gene expression in unions?

Authors:  Heidi Sutherland; Wendy A Bickmore
Journal:  Nat Rev Genet       Date:  2009-07       Impact factor: 53.242

5.  A modified fluorescence in situ hybridization protocol for Plasmodium falciparum greatly improves nuclear architecture conservation.

Authors:  Monica Contreras-Dominguez; Carolina Borsoi Moraes; Thierry Dorval; Auguste Genovesio; Fernando de Macedo Dossin; Lucio H Freitas-Junior
Journal:  Mol Biochem Parasitol       Date:  2010-04-28       Impact factor: 1.759

6.  Telomeric heterochromatin propagation and histone acetylation control mutually exclusive expression of antigenic variation genes in malaria parasites.

Authors:  Lucio H Freitas-Junior; Rosaura Hernandez-Rivas; Stuart A Ralph; Dvorak Montiel-Condado; Omar K Ruvalcaba-Salazar; Ana Paola Rojas-Meza; Liliana Mâncio-Silva; Ricardo J Leal-Silvestre; Alisson Marques Gontijo; Spencer Shorte; Artur Scherf
Journal:  Cell       Date:  2005-04-08       Impact factor: 41.582

7.  Genome-wide analysis of heterochromatin associates clonally variant gene regulation with perinuclear repressive centers in malaria parasites.

Authors:  Jose-Juan Lopez-Rubio; Liliana Mancio-Silva; Artur Scherf
Journal:  Cell Host Microbe       Date:  2009-02-19       Impact factor: 21.023

8.  Nuclear non-coding RNAs are transcribed from the centromeres of Plasmodium falciparum and are associated with centromeric chromatin.

Authors:  Felomena Li; Lakshmi Sonbuchner; Sue A Kyes; Christian Epp; Kirk W Deitsch
Journal:  J Biol Chem       Date:  2007-12-28       Impact factor: 5.157

9.  Differential association of Orc1 and Sir2 proteins to telomeric domains in Plasmodium falciparum.

Authors:  Liliana Mancio-Silva; Ana Paola Rojas-Meza; Miguel Vargas; Artur Scherf; Rosaura Hernandez-Rivas
Journal:  J Cell Sci       Date:  2008-06-15       Impact factor: 5.285

10.  Differential sub-nuclear localisation of repressive and activating histone methyl modifications in P. falciparum.

Authors:  Neha Issar; Stuart A Ralph; Liliana Mancio-Silva; Catherine Keeling; Artur Scherf
Journal:  Microbes Infect       Date:  2008-12-27       Impact factor: 2.700
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  4 in total

1.  Exonuclease-mediated degradation of nascent RNA silences genes linked to severe malaria.

Authors:  Qingfeng Zhang; T Nicolai Siegel; Rafael M Martins; Fei Wang; Jun Cao; Qi Gao; Xiu Cheng; Lubin Jiang; Chung-Chau Hon; Christine Scheidig-Benatar; Hiroshi Sakamoto; Louise Turner; Anja T R Jensen; Aurelie Claes; Julien Guizetti; Nicholas A Malmquist; Artur Scherf
Journal:  Nature       Date:  2014-06-29       Impact factor: 49.962

2.  Nuclear pores and perinuclear expression sites of var and ribosomal DNA genes correspond to physically distinct regions in Plasmodium falciparum.

Authors:  Julien Guizetti; Rafael Miyazawa Martins; Stéphanie Guadagnini; Aurélie Claes; Artur Scherf
Journal:  Eukaryot Cell       Date:  2013-03-08

3.  Modifications at K31 on the lateral surface of histone H4 contribute to genome structure and expression in apicomplexan parasites.

Authors:  Fabien Sindikubwabo; Shuai Ding; Tahir Hussain; Philippe Ortet; Mohamed Barakat; Sebastian Baumgarten; Dominique Cannella; Andrés Palencia; Alexandre Bougdour; Lucid Belmudes; Yohann Couté; Isabelle Tardieux; Cyrille Y Botté; Artur Scherf; Mohamed-Ali Hakimi
Journal:  Elife       Date:  2017-11-04       Impact factor: 8.140

4.  Trans-acting GC-rich non-coding RNA at var expression site modulates gene counting in malaria parasite.

Authors:  Julien Guizetti; Anna Barcons-Simon; Artur Scherf
Journal:  Nucleic Acids Res       Date:  2016-07-27       Impact factor: 16.971
  4 in total

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