Literature DB >> 2298371

Preparation and properties of brush-border membrane vesicles from human small intestine.

S P Shirazi-Beechey1, A G Davies, K Tebbutt, J Dyer, A Ellis, C J Taylor, P Fairclough, R B Beechey.   

Abstract

This study describes a simple and rapid method for the preparation of brush-border membrane vesicles from intestinal biopsies. The specific activities of sucrase, amino peptidase N, and alkaline phosphatase in these vesicles were the same as those in vesicles prepared from intestinal segments. The vesicles from all the regions of the small intestine can transport D-glucose in an Na+-dependent manner. The rates of transport of D-glucose presented here are far higher than previously reported. The method should have a wide applicability to studies of transport mechanisms and the distribution of transport processes within the intestine.

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Year:  1990        PMID： 2298371     DOI： 10.1016/0016-5085(90)90288-c

Source DB:  PubMed          Journal:  Gastroenterology        ISSN： 0016-5085            Impact factor:   22.682


  29 in total

1.  Nutrient regulation of human intestinal sugar transporter (SGLT1) expression.

Authors:  J Dyer; K B Hosie; S P Shirazi-Beechey
Journal:  Gut       Date:  1997-07       Impact factor: 23.059

2.  Isolation and characterization of renal cortical membranes using an aqueous two-phase partition technique.

Authors:  T G Hammond; R R Majewski; J J Onorato; P C Brazy; D J Morré
Journal:  Biochem J       Date:  1993-06-15       Impact factor: 3.857

3.  Sodium/glucose cotransporter activity in cystic fibrosis.

Authors:  P T Hardcastle; C J Taylor
Journal:  Arch Dis Child       Date:  1996-08       Impact factor: 3.791

4.  A simple method for measuring of intestinal solute transport in mucosal biopsy specimens.

Authors:  M Stelzner; S Somasundaram; D Kearney
Journal:  Dig Dis Sci       Date:  2001-03       Impact factor: 3.199

5.  Substrate-induced regulation of the human colonic monocarboxylate transporter, MCT1.

Authors:  Mark A Cuff; Daniel W Lambert; Soraya P Shirazi-Beechey
Journal:  J Physiol       Date:  2002-03-01       Impact factor: 5.182

6.  Luminal lipid regulates CD36 levels and downstream signaling to stimulate chylomicron synthesis.

Authors:  Thi Thu Trang Tran; Hélène Poirier; Lionel Clément; Fatiha Nassir; Maurice M A L Pelsers; Valérie Petit; Pascal Degrace; Marie-Claude Monnot; Jan F C Glatz; Nada A Abumrad; Philippe Besnard; Isabelle Niot
Journal:  J Biol Chem       Date:  2011-05-24       Impact factor: 5.157

7.  Characterisation of penicillin G uptake in human small intestinal brush border membrane vesicles.

Authors:  J F Poschet; S M Hammond; P D Fairclough
Journal:  Gut       Date:  1999-05       Impact factor: 23.059

8.  Identification and characterization of a monocarboxylate transporter (MCT1) in pig and human colon: its potential to transport L-lactate as well as butyrate.

Authors:  A Ritzhaupt; I S Wood; A Ellis; K B Hosie; S P Shirazi-Beechey
Journal:  J Physiol       Date:  1998-12-15       Impact factor: 5.182

9.  Adaptive response of equine intestinal Na+/glucose co-transporter (SGLT1) to an increase in dietary soluble carbohydrate.

Authors:  Jane Dyer; Miran Al-Rammahi; Louise Waterfall; Kieron S H Salmon; Ray J Geor; Ludovic Bouré; G Barrie Edwards; Christopher J Proudman; Soraya P Shirazi-Beechey
Journal:  Pflugers Arch       Date:  2008-12-02       Impact factor: 3.657

10.  Preparation and characterization of basolateral membrane vesicles from pig and human colonocytes: the mechanism of glucose transport.

Authors:  S A Pinches; S M Gribble; R B Beechey; A Ellis; J M Shaw; S P Shirazi-Beechey
Journal:  Biochem J       Date:  1993-09-01       Impact factor: 3.857
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