J F Poschet1, S M Hammond, P D Fairclough. 1. Digestive Disease Research Centre, St Bartholomew's and the Royal London School of Medicine and Dentistry, Turner Street, London E1 2AD, UK.
Abstract
BACKGROUND: Many beta lactams are well absorbed by the small intestine, although the reasons for this are poorly understood. AIMS: To characterise the uptake of penicillin G into human small intestinal brush border membrane vesicles (BBMV) and to compare the uptake characteristics to those of rabbit BBMV. METHODS AND RESULTS: Uptake of penicillin G was studied in human BBMV. Penicillin G was actively transported into the lumen of BBMV via an H+ dependent, Na+ independent uptake system. The carrier mediated process was saturable and adhered to Michaelis-Menten kinetics (Vmax 52 nmol penicillin G per mg protein per 30 seconds, Km 13.9 mM). These results are similar to those previously reported in rabbit BBMV. CONCLUSIONS: It is suggested that penicillin G can be used as a model molecule for peptide and beta lactam transport studies as it is cheap and readily available in isotopically labelled form. Furthermore, rabbit BBMV may be used as an acceptable substitute for human BBMV for the study of penicillin transport.
BACKGROUND: Many beta lactams are well absorbed by the small intestine, although the reasons for this are poorly understood. AIMS: To characterise the uptake of penicillin G into human small intestinal brush border membrane vesicles (BBMV) and to compare the uptake characteristics to those of rabbitBBMV. METHODS AND RESULTS: Uptake of penicillin G was studied in humanBBMV. Penicillin G was actively transported into the lumen of BBMV via an H+ dependent, Na+ independent uptake system. The carrier mediated process was saturable and adhered to Michaelis-Menten kinetics (Vmax 52 nmol penicillin G per mg protein per 30 seconds, Km 13.9 mM). These results are similar to those previously reported in rabbitBBMV. CONCLUSIONS: It is suggested that penicillin G can be used as a model molecule for peptide and beta lactam transport studies as it is cheap and readily available in isotopically labelled form. Furthermore, rabbitBBMV may be used as an acceptable substitute for humanBBMV for the study of penicillin transport.
Authors: S P Shirazi-Beechey; A G Davies; K Tebbutt; J Dyer; A Ellis; C J Taylor; P Fairclough; R B Beechey Journal: Gastroenterology Date: 1990-03 Impact factor: 22.682
Authors: Kaixun Li; Liya Xu; Ashutosh A Kulkarni; Daya I Perkins; Ian S Haworth; Daryl L Davies Journal: Alcohol Clin Exp Res Date: 2008-03-11 Impact factor: 3.455