Literature DB >> 22978241

Inhibition of essential bacterial peptidyl-tRNA hydrolase activity by tropical plant extracts.

Hana McFeeters1, Morgan J Gilbert, Rachel M Thompson, William N Setzer, Luis R Cruz-Vera, Robert L McFeeters.   

Abstract

Peptidyl-tRNA Hydrolase (Pth) is a highly conserved, essential enzyme in bacteria. It removes the peptide portion from peptidyl-tRNA, returning free tRNAs to participate in translation. Build-up of peptidyl-tRNAs is toxic and defects in Pth function result in cell death. Herein we use in vitro activity of recombinant E. coli Pth to screen tropical plant extracts for inhibition. Multiple extracts were found to have inhibitory activity with some exhibiting different inhibitory effects depending on extraction conditions. IC50 values ranged from 0.02 to > 53.8 microg of extract per 1 unit of Pth, holding promise for in vivo screening. The inhibitory components in these extracts may serve as lead compounds for development of novel antibacterials.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22978241

Source DB:  PubMed          Journal:  Nat Prod Commun        ISSN： 1555-9475            Impact factor:   0.986


  8 in total

1.  Neutron diffraction analysis of Pseudomonas aeruginosa peptidyl-tRNA hydrolase 1.

Authors:  Hana McFeeters; Venu Gopal Vandavasi; Kevin L Weiss; Leighton Coates; Robert L McFeeters
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2016-02-19       Impact factor: 1.056

2.  Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium.

Authors:  Venugopal Vandavasi; Kasey Taylor-Creel; Robert L McFeeters; Leighton Coates; Hana McFeeters
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-06-18       Impact factor: 1.056

3.  Bacterial production of site specific 13C labeled phenylalanine and methodology for high level incorporation into bacterially expressed recombinant proteins.

Authors:  Bhargavi Ramaraju; Hana McFeeters; Bernhard Vogler; Robert L McFeeters
Journal:  J Biomol NMR       Date:  2016-12-27       Impact factor: 2.835

4.  Small molecule binding, docking, and characterization of the interaction between Pth1 and peptidyl-tRNA.

Authors:  Mary C Hames; Hana McFeeters; W Blake Holloway; Christopher B Stanley; Volker S Urban; Robert L McFeeters
Journal:  Int J Mol Sci       Date:  2013-11-19       Impact factor: 5.923

5.  Small Molecule Docking Supports Broad and Narrow Spectrum Potential for the Inhibition of the Novel Antibiotic Target Bacterial Pth1.

Authors:  Paul P Ferguson; W Blake Holloway; William N Setzer; Hana McFeeters; Robert L McFeeters
Journal:  Antibiotics (Basel)       Date:  2016-05-10

6.  Novel Antifungal Activity for the Lectin Scytovirin: Inhibition of Cryptococcus neoformans and Cryptococcus gattii.

Authors:  Tyler H Jones; Erin E McClelland; Hana McFeeters; Robert L McFeeters
Journal:  Front Microbiol       Date:  2017-05-09       Impact factor: 5.640

7.  Expedited isolation of natural product peptidyl-tRNA hydrolase inhibitors from a Pth1 affinity column.

Authors:  Harkirat S Sethi; Jessica L Osier; Geordan L Burks; Jennifer F Lamar; Hana McFeeters; Robert L McFeeters
Journal:  AIMS Mol Sci       Date:  2017-05-12

8.  Natural Product Inhibition and Enzyme Kinetics Related to Phylogenetic Characterization for Bacterial Peptidyl-tRNA Hydrolase 1.

Authors:  D Scott Strange; Steven S Gaffin; W Blake Holloway; Meredyth D Kinsella; Jacob N Wisotsky; Hana McFeeters; Robert L McFeeters
Journal:  Molecules       Date:  2021-04-15       Impact factor: 4.411
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.