OBJECTIVE: To identify and quantitatively determine Mesenchymal stem cells (MSCs) in the umbilical cord blood (UCB) of neonates born at different gestational periods. METHODS: UCB was collected at birth in neonates of three different gestational groups. The mononuclear cells (MNCs) were phenotypically analyzed by flow cytometer. RESULTS: The yield of total MNCs did not differ much with gestation; the average values were 22.6 ± 6.48 × 106 cells/ml. The MSCs were significantly higher in the lower gestation group. These were 0.0219 ± 0.012 %, 0.0044 ± 0.003 % and 0.0022 ± 0.003 % in 28 to 31 wk, 32 to 35 wk and >36 wk, respectively (P = 0.00). There was a significant inverse correlation between the gestational age and the presence of MSCs with a correlation co-efficient of -0.54 (P = 0.0001). CONCLUSIONS: The MSCs population was significantly higher in infants born at lesser gestation than those born at term gestation.
OBJECTIVE: To identify and quantitatively determine Mesenchymal stem cells (MSCs) in the umbilical cord blood (UCB) of neonates born at different gestational periods. METHODS: UCB was collected at birth in neonates of three different gestational groups. The mononuclear cells (MNCs) were phenotypically analyzed by flow cytometer. RESULTS: The yield of total MNCs did not differ much with gestation; the average values were 22.6 ± 6.48 × 106 cells/ml. The MSCs were significantly higher in the lower gestation group. These were 0.0219 ± 0.012 %, 0.0044 ± 0.003 % and 0.0022 ± 0.003 % in 28 to 31 wk, 32 to 35 wk and >36 wk, respectively (P = 0.00). There was a significant inverse correlation between the gestational age and the presence of MSCs with a correlation co-efficient of -0.54 (P = 0.0001). CONCLUSIONS: The MSCs population was significantly higher in infants born at lesser gestation than those born at term gestation.
Authors: Qizhou Lian; Elias Lye; Keng Suan Yeo; Eileen Khia Way Tan; Manuel Salto-Tellez; Tong Ming Liu; Nallasivam Palanisamy; Reida Menshawe El Oakley; Eng Hin Lee; Bing Lim; Sai-Kiang Lim Journal: Stem Cells Date: 2006-10-19 Impact factor: 6.277
Authors: G F Sanz; S Saavedra; C Jiménez; L Senent; J Cervera; D Planelles; P Bolufer; L Larrea; G Martín; J Martínez; I Jarque; F Moscardó; G Plumé; R Andreu; J de la Rubia; E Barragán; P Solves; M A Soler; M A Sanz Journal: Bone Marrow Transplant Date: 2001-04 Impact factor: 5.483
Authors: Jingang Li; Tamara Yawno; Amy Sutherland; Jan Loose; Ilias Nitsos; Beth J Allison; Robert Bischof; Courtney A McDonald; Graham Jenkin; Suzanne L Miller Journal: Pediatr Res Date: 2017-08-16 Impact factor: 3.756