Literature DB >> 2296288

Redesign of the coenzyme specificity of a dehydrogenase by protein engineering.

N S Scrutton1, A Berry, R N Perham.   

Abstract

Directed mutagenesis and molecular modelling have been used to identify a set of amino-acid side chains in glutathione reductase that confer specificity for the coenzyme NADP+. Systematic replacement of these amino acids, all of which occur in a 'fingerprint' structural motif in the NADP+-binding domain, leaves the substrate specificity unchanged but converts the enzyme into one displaying a marked preference for the coenzyme NAD+.

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Year:  1990        PMID： 2296288     DOI： 10.1038/343038a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  160 in total

1.  Biosynthetic gene cluster of simocyclinone, a natural multihybrid antibiotic.

Authors:  A Trefzer; S Pelzer; J Schimana; S Stockert; C Bihlmaier; H-P Fiedler; K Welzel; A Vente; A Bechthold
Journal:  Antimicrob Agents Chemother       Date:  2002-05       Impact factor: 5.191

2.  Cloning and sequence of the human adrenodoxin reductase gene.

Authors:  D Lin; Y F Shi; W L Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

3.  Genes coding for hepatotoxic heptapeptides (microcystins) in the cyanobacterium Anabaena strain 90.

Authors:  Leo Rouhiainen; Tanja Vakkilainen; Berit Lumbye Siemer; William Buikema; Robert Haselkorn; Kaarina Sivonen
Journal:  Appl Environ Microbiol       Date:  2004-02       Impact factor: 4.792

4.  Engineering of coenzyme specificity of formate dehydrogenase from Saccharomyces cerevisiae.

Authors:  Alexander E Serov; Anna S Popova; Vladimir V Fedorchuk; Vladimir I Tishkov
Journal:  Biochem J       Date:  2002-11-01       Impact factor: 3.857

5.  Cloning, sequencing, and functional analysis of an iterative type I polyketide synthase gene cluster for biosynthesis of the antitumor chlorinated polyenone neocarzilin in "Streptomyces carzinostaticus".

Authors:  Miyuki Otsuka; Koji Ichinose; Isao Fujii; Yutaka Ebizuka
Journal:  Antimicrob Agents Chemother       Date:  2004-09       Impact factor: 5.191

6.  Substrate specificity of trypsin investigated by using a genetic selection.

Authors:  L B Evnin; J R Vásquez; C S Craik
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

7.  Engineering the respiratory complex I to energy-converting NADPH:ubiquinone oxidoreductase.

Authors:  Klaudia Morina; Marius Schulte; Florian Hubrich; Katerina Dörner; Stefan Steimle; Stefan Stolpe; Thorsten Friedrich
Journal:  J Biol Chem       Date:  2011-08-10       Impact factor: 5.157

8.  Cloning and molecular analysis of two different ILV5 genes from a brewing strain of Saccharomyces cerevisiae.

Authors:  Q Xie; A Jiménez
Journal:  Curr Genet       Date:  1994 Nov-Dec       Impact factor: 3.886

9.  Structural prototypes for an extended family of flavoprotein reductases: comparison of phthalate dioxygenase reductase with ferredoxin reductase and ferredoxin.

Authors:  C C Correll; M L Ludwig; C M Bruns; P A Karplus
Journal:  Protein Sci       Date:  1993-12       Impact factor: 6.725

10.  Biochemical and structural studies of NADH-dependent FabG used to increase the bacterial production of fatty acids under anaerobic conditions.

Authors:  Pouya Javidpour; Jose H Pereira; Ee-Been Goh; Ryan P McAndrew; Suzanne M Ma; Gregory D Friedland; Jay D Keasling; Swapnil R Chhabra; Paul D Adams; Harry R Beller
Journal:  Appl Environ Microbiol       Date:  2013-11-08       Impact factor: 4.792
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