OBJECTIVE: To study H19 ICR methylation levels in association with sperm parameters routinely analyzed in idiopathic recurrent spontaneous miscarriage cases. DESIGN: Case-control study. SETTING: Academic research setting. PATIENT(S): Male partners of couples with a history of idiopathic recurrent spontaneous miscarriage (RSM group) and male partners of couples with proven fertility (control group). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Paternal age, sperm concentration, motility, chromatin compaction status, morphology, and H19 ICR methylation were assessed in control and idiopathic RSM group participants. RESULT(S): Paternal age and basic semen parameters analyzed did not show any significant difference between the two groups; however H19 ICR methylation levels were reduced significantly in the idiopathic RSM group compared with the control group. CONCLUSION(S): Significant reduction in the H19 ICR methylation without significant difference in the sperm parameters demonstrates aberrant imprinting to be associated with idiopathic RSM.
OBJECTIVE: To study H19 ICR methylation levels in association with sperm parameters routinely analyzed in idiopathic recurrent spontaneous miscarriage cases. DESIGN: Case-control study. SETTING: Academic research setting. PATIENT(S): Male partners of couples with a history of idiopathic recurrent spontaneous miscarriage (RSM group) and male partners of couples with proven fertility (control group). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Paternal age, sperm concentration, motility, chromatin compaction status, morphology, and H19 ICR methylation were assessed in control and idiopathic RSM group participants. RESULT(S): Paternal age and basic semen parameters analyzed did not show any significant difference between the two groups; however H19 ICR methylation levels were reduced significantly in the idiopathic RSM group compared with the control group. CONCLUSION(S): Significant reduction in the H19 ICR methylation without significant difference in the sperm parameters demonstrates aberrant imprinting to be associated with idiopathic RSM.
Authors: Ruth Kläver; Victoria Sánchez; Oliver S Damm; Klaus Redmann; Elisabeth Lahrmann; Reinhild Sandhowe-Klaverkamp; Christian Rohde; Joachim Wistuba; Jens Ehmcke; Stefan Schlatt; Jörg Gromoll Journal: PLoS One Date: 2015-02-18 Impact factor: 3.240
Authors: Bolan Yu; Hua Zhou; Min Liu; Ting Zheng; Lu Jiang; Mei Zhao; Xiaoxie Xu; Zhaofeng Huang Journal: PLoS One Date: 2015-12-28 Impact factor: 3.240
Authors: Karl T Kelsey; Matthew Rytel; Edward Dere; Rondi Butler; Melissa Eliot; Susan M Huse; E Andres Houseman; Devin C Koestler; Kim Boekelheide Journal: Environ Health Date: 2019-10-29 Impact factor: 5.984