Literature DB >> 22950739

Online nanoscale ERLIC-MS outperforms RPLC-MS for shotgun proteomics in complex mixtures.

Ebbing P de Jong1, Timothy J Griffin.   

Abstract

We have explored the use of electrostatic repulsion hydrophilic interaction chromatography (ERLIC) as an alternative to the gold-standard in shotgun proteomics: reversed-phase (RP) LC for online ESI-MS/MS. Conditions for sample solubilization and initial gradient conditions were optimized to strike a balance between peptide solubility and maximum peptide retention when using mobile phase with high organic solvent concentration. Online ERLIC-MS demonstrated a 57% increase in total peptide identifications compared to RP-MS. We examined the mechanism of this improved performance and found that it stems from ERLIC's propensity to retain longer peptides, which can be identified with greater confidence. Online nanoscale ERLIC-MS provides a powerful new tool for enhancing MS-based shotgun proteomic in a broad range of applications.

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Year:  2012        PMID： 22950739      PMCID： PMC3487405          DOI： 10.1021/pr300638n

Source DB:  PubMed          Journal:  J Proteome Res        ISSN： 1535-3893            Impact factor:   4.466


  19 in total

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Review 4.  Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples.

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7.  Genome-scale proteome quantification by DEEP SEQ mass spectrometry.

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  7 in total

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