AIM: Locoregional recurrences and distant metastases in adequately treated head and neck squamous cell carcinoma (HNSCC) patients have a dismal effect on survival. Tumor cells that escape histopathological detection might be the prime cause of this effect. We evaluated whether minimal residual cancer (MRC) in deep surgical margins and disseminated tumor cells (DTCs) in bone marrow aspirates are associated with clinicohistopathological parameters and outcome. METHODS: Submucosal samples of deep resection margins of 105 HNSCC patients with histopathologically tumor-free surgical margins were analysed for the presence of MRC using hLy-6D qRT-PCR. Bone-marrow aspirates of 76 of these patients were analysed for DTCs by immunocytochemical staining. Presence of molecular-positive deep surgical margins, presence of DTC in bone marrow aspirates, and clinicohistopathological parameters were tested for associations with survival parameters by univariate and multivariate analyses. RESULTS: In addition to lymph node stage, it appeared that vasoinvasive growth and particularly infiltrative growth pattern are significant predictors for locoregional recurrence (p = 0.041 and p = 0.006, respectively) and disease-free survival (p = 0.014 and p = 0.008, respectively). Remarkably, neither the presence of molecular-positive deep surgical margins nor that of DTC in bone marrow aspirates were significantly related to outcome. CONCLUSIONS: The presence of vasoinvasive and infiltrative growth in HNSCC tumor specimens are significant risk-factors for locoregional recurrence and disease-free survival. At present there seems no role for molecular analysis of deep surgical margins and bone marrow aspirates in predicting outcome with the methods used.
AIM: Locoregional recurrences and distant metastases in adequately treated head and neck squamous cell carcinoma (HNSCC) patients have a dismal effect on survival. Tumor cells that escape histopathological detection might be the prime cause of this effect. We evaluated whether minimal residual cancer (MRC) in deep surgical margins and disseminated tumor cells (DTCs) in bone marrow aspirates are associated with clinicohistopathological parameters and outcome. METHODS: Submucosal samples of deep resection margins of 105 HNSCCpatients with histopathologically tumor-free surgical margins were analysed for the presence of MRC using hLy-6D qRT-PCR. Bone-marrow aspirates of 76 of these patients were analysed for DTCs by immunocytochemical staining. Presence of molecular-positive deep surgical margins, presence of DTC in bone marrow aspirates, and clinicohistopathological parameters were tested for associations with survival parameters by univariate and multivariate analyses. RESULTS: In addition to lymph node stage, it appeared that vasoinvasive growth and particularly infiltrative growth pattern are significant predictors for locoregional recurrence (p = 0.041 and p = 0.006, respectively) and disease-free survival (p = 0.014 and p = 0.008, respectively). Remarkably, neither the presence of molecular-positive deep surgical margins nor that of DTC in bone marrow aspirates were significantly related to outcome. CONCLUSIONS: The presence of vasoinvasive and infiltrative growth in HNSCC tumor specimens are significant risk-factors for locoregional recurrence and disease-free survival. At present there seems no role for molecular analysis of deep surgical margins and bone marrow aspirates in predicting outcome with the methods used.
Authors: Fania S Doekhie; Wilma E Mesker; J Han J M van Krieken; Niels F M Kok; Henk H Hartgrink; Elma Klein Kranenbarg; Hein Putter; Peter J K Kuppen; Hans J Tanke; Rob A E M Tollenaar; Cornelis J H van de Velde Journal: Am J Surg Pathol Date: 2005-09 Impact factor: 6.394
Authors: Boudewijn J M Braakhuis; Maarten P Tabor; J Alain Kummer; C René Leemans; Ruud H Brakenhoff Journal: Cancer Res Date: 2003-04-15 Impact factor: 12.701
Authors: A Peggy Graveland; Pawel J Golusinski; Marijke Buijze; Rinze Douma; Nanet Sons; Dirk J Kuik; Elisabeth Bloemena; C René Leemans; Ruud H Brakenhoff; Boudewijn J M Braakhuis Journal: Int J Cancer Date: 2011-04-15 Impact factor: 7.396
Authors: Eline J C Nieuwenhuis; C René Leemans; J Alain Kummer; Fedor Denkers; Gordon B Snow; Ruud H Brakenhoff; Alain Kummer Journal: Lab Invest Date: 2003-08 Impact factor: 5.662
Authors: A Peggy Graveland; Michiel de Maaker; Boudewijn J M Braakhuis; Remco de Bree; Simone E J Eerenstein; Elisabeth Bloemena; C René Leemans; Ruud H Brakenhoff Journal: Cell Oncol Date: 2009 Impact factor: 6.730
Authors: Matthew L Hedberg; Gerald Goh; Simion I Chiosea; Julie E Bauman; Maria L Freilino; Yan Zeng; Lin Wang; Brenda B Diergaarde; William E Gooding; Vivian W Y Lui; Roy S Herbst; Richard P Lifton; Jennifer R Grandis Journal: J Clin Invest Date: 2015-11-30 Impact factor: 14.808
Authors: Jennifer L Hunt; Leon Barnes; James S Lewis; Magdy E Mahfouz; Pieter J Slootweg; Lester D R Thompson; Antonio Cardesa; Kenneth O Devaney; Douglas R Gnepp; William H Westra; Juan P Rodrigo; Julia A Woolgar; Alessandra Rinaldo; Asterios Triantafyllou; Robert P Takes; Alfio Ferlito Journal: Eur Arch Otorhinolaryngol Date: 2013-03-07 Impact factor: 2.503
Authors: Maria J De Herdt; Stefan M Willems; Berdine van der Steen; Rob Noorlag; Esther I Verhoef; Geert J L H van Leenders; Robert J J van Es; Senada KoljenoviÄ; Robert J Baatenburg de Jong; Leendert H J Looijenga Journal: Oncotarget Date: 2016-03-15