Literature DB >> 22932771

Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides.

Vanessa Siegmund1, Tobias Santner, Ronald Micura, Andreas Marx.   

Abstract

We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2'-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2'-methylseleno-2'-deoxyuridine 5'-triphosphate.

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Year:  2012        PMID： 22932771     DOI： 10.1039/c2cc35028a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  9 in total

Review 1.  In vitro selection using modified or unnatural nucleotides.

Authors:  Scott M Knudsen; Michael P Robertson; Andrew D Ellington
Journal:  Curr Protoc Nucleic Acid Chem       Date:  2002-02

Review 2.  In vitro selection using modified or unnatural nucleotides.

Authors:  Gwendolyn M Stovall; Robert S Bedenbaugh; Shruti Singh; Adam J Meyer; Paul J Hatala; Andrew D Ellington; Bradley Hall
Journal:  Curr Protoc Nucleic Acid Chem       Date:  2014-03-26

3.  In vitro RNA SELEX for the generation of chemically-optimized therapeutic RNA drugs.

Authors:  Kevin T Urak; Sabrina Shore; William M Rockey; Shi-Jie Chen; Anton P McCaffrey; Paloma H Giangrande
Journal:  Methods       Date:  2016-03-10       Impact factor: 3.608

4.  Transcription yield of fully 2'-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants.

Authors:  Adam J Meyer; Daniel J Garry; Bradley Hall; Michelle M Byrom; Hannah G McDonald; Xu Yang; Y Whitney Yin; Andrew D Ellington
Journal:  Nucleic Acids Res       Date:  2015-07-24       Impact factor: 16.971

5.  Discovery of a capuramycin analog that kills nonreplicating Mycobacterium tuberculosis and its synergistic effects with translocase I inhibitors.

Authors:  Shajila Siricilla; Katsuhiko Mitachi; Bajoie Wan; Scott G Franzblau; Michio Kurosu
Journal:  J Antibiot (Tokyo)       Date:  2014-10-01       Impact factor: 2.649

Review 6.  Nucleic Acid Aptamers: Emerging Applications in Medical Imaging, Nanotechnology, Neurosciences, and Drug Delivery.

Authors:  Pascal Röthlisberger; Cécile Gasse; Marcel Hollenstein
Journal:  Int J Mol Sci       Date:  2017-11-16       Impact factor: 5.923

Review 7.  In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies.

Authors:  Farhana Lipi; Suxiang Chen; Madhuri Chakravarthy; Shilpa Rakesh; Rakesh N Veedu
Journal:  RNA Biol       Date:  2016-10-07       Impact factor: 4.652

Review 8.  Exploring the Chemistry of Genetic Information Storage and Propagation through Polymerase Engineering.

Authors:  Gillian Houlihan; Sebastian Arangundy-Franklin; Philipp Holliger
Journal:  Acc Chem Res       Date:  2017-04-06       Impact factor: 22.384

9.  Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity.

Authors:  Priya Kalra; Abhijeet Dhiman; William C Cho; John G Bruno; Tarun K Sharma
Journal:  Front Mol Biosci       Date:  2018-05-14
  9 in total

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