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Literature DB >> 22911502

New pulsed field gradient NMR experiments for the detection of bound water in proteins.

Abstract

New H(2)O-selective homonuclear and heteronuclear 2D NMR experiments have been designed for the observation of protein hydration (PHOGSY, Protein Hydration Observed by Gradient Spectroscop Y). These experiments utilize selective excitation of the H(2)O resonance and pulsed field gradients for coherence selection and efficient H(2)O suppression. The method allows for a rapid and sensitive detection of H(2)O molecules in labelled and unlabelled proteins. In addition it opens a way to measure the residence time of water bound to proteins. Its application to uniformly (15)N-labelled FKBP-12 (FK-506 binding protein) is demonstrated.

Entities: Chemical Gene

Year: 1995 PMID： 22911502 DOI： 10.1007/BF00211757

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

19 in total

1. Nuclear magnetic resonance detection of bound water molecules in the active site of Lactobacillus casei dihydrofolate reductase in aqueous solution.

Authors: I P Gerothanassis; B Birdsall; C J Bauer; T A Frenkiel; J Feeney
Journal: J Mol Biol Date: 1992-07-20 Impact factor: 5.469

2. Three-dimensional NMR spectroscopy of a protein in solution.

Authors: H Oschkinat; C Griesinger; P J Kraulis; O W Sørensen; R R Ernst; A M Gronenborn; G M Clore
Journal: Nature Date: 1988-03-24 Impact factor: 49.962

Review 3. Water: now you see it, now you don't.

Authors: M Levitt; B H Park
Journal: Structure Date: 1993-12-15 Impact factor: 5.006

4. Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.

Authors: D Marion; P C Driscoll; L E Kay; P T Wingfield; A Bax; A M Gronenborn; G M Clore
Journal: Biochemistry Date: 1989-07-25 Impact factor: 3.162

5. Water-inserted alpha-helical segments implicate reverse turns as folding intermediates.

Authors: M Sundaralingam; Y C Sekharudu
Journal: Science Date: 1989-06-16 Impact factor: 47.728

6. Identification and localization of bound internal water in the solution structure of interleukin 1 beta by heteronuclear three-dimensional 1H rotating-frame Overhauser 15N-1H multiple quantum coherence NMR spectroscopy.

Authors: G M Clore; A Bax; P T Wingfield; A M Gronenborn
Journal: Biochemistry Date: 1990-06-19 Impact factor: 3.162

7. Heteronuclear 3D NMR studies of water bound to an FK506 binding protein/immunosuppressant complex.

Authors: R X Xu; R P Meadows; S W Fesik
Journal: Biochemistry Date: 1993-03-16 Impact factor: 3.162

8. Localization of bound water in the solution structure of the immunoglobulin binding domain of streptococcal protein G. Evidence for solvent-induced helical distortion in solution.

Authors: G M Clore; A M Gronenborn
Journal: J Mol Biol Date: 1992-02-20 Impact factor: 5.469

4. Accurate quantitation of water-amide proton exchange rates using the phase-modulated CLEAN chemical EXchange (CLEANEX-PM) approach with a Fast-HSQC (FHSQC) detection scheme.

Authors: T L Hwang; P C van Zijl; S Mori
Journal: J Biomol NMR Date: 1998-02 Impact factor: 2.835

5. Investigating the dynamic aspects of drug-protein recognition through a combination of MD and NMR analyses: implications for the development of protein-protein interaction inhibitors.

Authors: Massimiliano Meli; Katiuscia Pagano; Laura Ragona; Giorgio Colombo
Journal: PLoS One Date: 2014-05-27 Impact factor: 3.240

5 in total

New pulsed field gradient NMR experiments for the detection of bound water in proteins.

1. Nuclear magnetic resonance detection of bound water molecules in the active site of Lactobacillus casei dihydrofolate reductase in aqueous solution.

2. Three-dimensional NMR spectroscopy of a protein in solution.

Review 3. Water: now you see it, now you don't.

4. Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.

5. Water-inserted alpha-helical segments implicate reverse turns as folding intermediates.

6. Identification and localization of bound internal water in the solution structure of interleukin 1 beta by heteronuclear three-dimensional 1H rotating-frame Overhauser 15N-1H multiple quantum coherence NMR spectroscopy.

7. Heteronuclear 3D NMR studies of water bound to an FK506 binding protein/immunosuppressant complex.

8. Localization of bound water in the solution structure of the immunoglobulin binding domain of streptococcal protein G. Evidence for solvent-induced helical distortion in solution.

9. Structural water in oxidized and reduced horse heart cytochrome c.

10. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

1. Topology and immersion depth of an integral membrane protein by paramagnetic rates from dissolved oxygen.

2. Water-macromolecule interactions by NMR: a quadrature-free constant-time approach and its application to CI2.

3. Parameterization of solvent-protein interaction and its use on NMR protein structure determination.

4. Accurate quantitation of water-amide proton exchange rates using the phase-modulated CLEAN chemical EXchange (CLEANEX-PM) approach with a Fast-HSQC (FHSQC) detection scheme.

5. Investigating the dynamic aspects of drug-protein recognition through a combination of MD and NMR analyses: implications for the development of protein-protein interaction inhibitors.