Literature DB >> 22907777

In vivo analysis of the morphologic characteristics of synthetic mesh to resist MRSA adherence.

Jeffrey A Blatnik1, David M Krpata, Michael R Jacobs, Yue Gao, Yuri W Novitsky, Michael J Rosen.   

Abstract

BACKGROUND: Synthetic mesh has significantly reduced recurrence rates for ventral hernia repair; however, prosthetic mesh infections remain a significant complication. We hypothesized that unique mesh constructs might alter the ability of various synthetic meshes to clear bacterial contamination. To evaluate this, we studied commercially available synthetic meshes ability to clear a bacterial contamination with methicillin resistant Staphylococcus aureus infection.
METHODS: Two hundred and eighty-three rats underwent hernia repair with one of nine synthetic materials. Control animals were closed, and the remainder was inoculated with either 10(4) or 10(6) methicillin-resistant S. aureus (MRSA). Animals were survived for 30 days without systemic antibiotics. At necropsy, the mesh was harvested and quantitative cultures and bacterial clearance assessed.
RESULTS: All clean repairs remained sterile. Rates of bacterial clearance for 10(4) repairs revealed that unprotected monofilament materials cleared significantly more bacteria than composite meshes and multifilament meshes (p = <0.01 and p = 0.01, respectively). At higher levels of bacterial contamination (10(6)), all materials had a reduction in bacterial clearance, although monofilament materials had higher bacterial clearance compared to composite meshes (p = 0.03).
CONCLUSIONS: Monofilament unprotected polypropylene and polyester mesh can clear a large percentage of MRSA contaminants. Multifilament, composite anti-adhesive barrier meshes, and laminar antimicrobial impregnated mesh are not able to clear bacterial contamination with MRSA. Unique properties of synthetic material should be considered when evaluating a prosthetic for high-risk incisional hernia repair.

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Year:  2012        PMID: 22907777     DOI: 10.1007/s11605-012-1992-5

Source DB:  PubMed          Journal:  J Gastrointest Surg        ISSN: 1091-255X            Impact factor:   3.452


  13 in total

1.  A comparison of suture repair with mesh repair for incisional hernia.

Authors:  R W Luijendijk; W C Hop; M P van den Tol; D C de Lange; M M Braaksma; J N IJzermans; R U Boelhouwer; B C de Vries; M K Salu; J C Wereldsma; C M Bruijninckx; J Jeekel
Journal:  N Engl J Med       Date:  2000-08-10       Impact factor: 91.245

2.  Susceptibility of prosthetic biomaterials to infection.

Authors:  A M Carbonell; K W Kercher; R F Sing; B T Heniford
Journal:  Surg Endosc       Date:  2005-09-30       Impact factor: 4.584

Review 3.  The phenomenon of infection with abdominal wall reconstruction.

Authors:  Anton F Engelsman; Henny C van der Mei; Rutger J Ploeg; Henk J Busscher
Journal:  Biomaterials       Date:  2007-02-02       Impact factor: 12.479

Review 4.  Concise review of mechanisms of bacterial adhesion to biomaterial surfaces.

Authors:  Y H An; R J Friedman
Journal:  J Biomed Mater Res       Date:  1998

5.  Novel in vitro model for assessing susceptibility of synthetic hernia repair meshes to Staphylococcus aureus infection using green fluorescent protein-labeled bacteria and modern imaging techniques.

Authors:  Ihab Halaweish; Karem Harth; Ann-Marie Broome; Gabriela Voskerician; Michael R Jacobs; Michael J Rosen
Journal:  Surg Infect (Larchmt)       Date:  2010-10       Impact factor: 2.150

6.  Initial adhesion and surface growth of Staphylococcus epidermidis and Pseudomonas aeruginosa on biomedical polymers.

Authors:  B Gottenbos; H C van der Mei; H J Busscher
Journal:  J Biomed Mater Res       Date:  2000-05

7.  In vitro infectability of prosthetic mesh by methicillin-resistant Staphylococcus aureus.

Authors:  A G Harrell; Y W Novitsky; K W Kercher; M Foster; J M Burns; T S Kuwada; B T Heniford
Journal:  Hernia       Date:  2006-02-02       Impact factor: 4.739

8.  Functional and morphological evaluation of a low-weight, monofilament polypropylene mesh for hernia repair.

Authors:  U Klinge; K Junge; M Stumpf; A P Ottinger AP; B Klosterhalfen
Journal:  J Biomed Mater Res       Date:  2002

9.  Long-term follow-up of a randomized controlled trial of suture versus mesh repair of incisional hernia.

Authors:  Jacobus W A Burger; Roland W Luijendijk; Wim C J Hop; Jens A Halm; Emiel G G Verdaasdonk; Johannes Jeekel
Journal:  Ann Surg       Date:  2004-10       Impact factor: 12.969

10.  Optimisation of polymeric surface pre-treatment to prevent bacterial biofilm formation for use in microfluidics.

Authors:  Colin A B Davidson; Christopher R Lowe
Journal:  J Mol Recognit       Date:  2004 May-Jun       Impact factor: 2.137

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  16 in total

1.  Development of a novel murine model for treatment of infected mesh scenarios.

Authors:  Arnab Majumder; Clayton C Petro; Lijia Liu; Mojtaba Fayezizadeh; Yuri W Novitsky
Journal:  Surg Endosc       Date:  2016-06-28       Impact factor: 4.584

Review 2.  A critical review of the in vitro and in vivo models for the evaluation of anti-infective meshes.

Authors:  O Guillaume; B Pérez Kohler; R Fortelny; H Redl; F Moriarty; R G Richards; D Eglin; A Petter Puchner
Journal:  Hernia       Date:  2018-08-28       Impact factor: 4.739

3.  A retrospective review and observations over a 16-year clinical experience on the surgical treatment of chronic mesh infection. What about replacing a synthetic mesh on the infected surgical field?

Authors:  C Birolini; J S de Miranda; E M Utiyama; S Rasslan
Journal:  Hernia       Date:  2014-02-09       Impact factor: 4.739

4.  Management of ventral hernia defect during enterocutaneous fistula takedown: practice patterns and short-term outcomes from the Abdominal Core Health Quality Collaborative.

Authors:  A Fafaj; L Tastaldi; H Alkhatib; S J Zolin; S Rosenblatt; L-C Huang; S Phillips; D M Krpata; A S Prabhu; C C Petro; M J Rosen
Journal:  Hernia       Date:  2021-01-02       Impact factor: 4.739

5.  Gentamicin for prevention of intraoperative mesh contamination: demonstration of high bactericide effect (in vitro) and low systemic bioavailability (in vivo).

Authors:  A Wiegering; B Sinha; L Spor; U Klinge; U Steger; C T Germer; U A Dietz
Journal:  Hernia       Date:  2014-08-12       Impact factor: 4.739

Review 6.  Synthetic Mesh in Contaminated Abdominal Wall Surgery: Friend or Foe? A Literature Review.

Authors:  Emmanuel E Sadava; Camila Bras Harriott; Cristian A Angeramo; Francisco Schlottmann
Journal:  J Gastrointest Surg       Date:  2021-09-29       Impact factor: 3.452

7.  Central failures of lightweight monofilament polyester mesh causing hernia recurrence: a cautionary note.

Authors:  C C Petro; E H Nahabet; C N Criss; S B Orenstein; H A von Recum; Y W Novitsky; M J Rosen
Journal:  Hernia       Date:  2015-02       Impact factor: 4.739

8.  An experimental comparison of the effects of bacterial colonization on biologic and synthetic meshes.

Authors:  William C Cole; Eric M Balent; Pamela C Masella; Lauren N Kajiura; Karen W Matsumoto; Lisa M Pierce
Journal:  Hernia       Date:  2014-08-01       Impact factor: 4.739

9.  Mesh cancer: long-term mesh infection leading to squamous-cell carcinoma of the abdominal wall.

Authors:  C Birolini; J G Minossi; C F Lima; E M Utiyama; S Rasslan
Journal:  Hernia       Date:  2013-04-19       Impact factor: 4.739

10.  Infection prevention using affinity polymer-coated, synthetic meshes in a pig hernia model.

Authors:  Jeffrey A Blatnik; Thimma R Thatiparti; David M Krpata; Sean T Zuckerman; Michael J Rosen; Horst A von Recum
Journal:  J Surg Res       Date:  2017-05-08       Impact factor: 2.192

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