Kang-Sheng Gu1, Yu Chen. 1. Department of Oncology, the Fist Affiliated Hospital of Anhui Medical University, Hefei, China. gukangsheng@yeah.net
Abstract
OBJECTIVE: To investigate possible signal pathway involvement in multi-drug resistant P-glycoprotein (P-gp) expression induced by cyclooxygenase-2 (COX-2) in a human gastric adenocarcinoma cell line stimulated with pacliaxel (TAX). METHODS: The effects of TAX on SGC7901 cell growth with different doses was assessed by MTT assay, along with the effects of the COX-2 selective inhibitor NS-398 and the nuclear factor-KB (NF-KB) pathway inhibitor pyrrolidine dithiocarbamate (PDTC). Influence on COX-2, NF-KB p65 and P-gp expression was determined by Western blotting. RESULTS: TAX, NS-398 and PDTC all reduced SGC7901 growth, with dose- dependence. With increasing dose of TAX, the expression of COX-2, p65 and P-gp showed rising trends, this being reversed by NS-398. PDTC also caused decrease in expression of p65 and P-gp over time. CONCLUSION: COX-2 may induce the expression of P-gp in SGC7901 cell line via the NF-kappa B pathway with pacliaxel stimulation.
OBJECTIVE: To investigate possible signal pathway involvement in multi-drug resistant P-glycoprotein (P-gp) expression induced by cyclooxygenase-2 (COX-2) in a humangastric adenocarcinoma cell line stimulated with pacliaxel (TAX). METHODS: The effects of TAX on SGC7901 cell growth with different doses was assessed by MTT assay, along with the effects of the COX-2 selective inhibitor NS-398 and the nuclear factor-KB (NF-KB) pathway inhibitor pyrrolidine dithiocarbamate (PDTC). Influence on COX-2, NF-KB p65 and P-gp expression was determined by Western blotting. RESULTS: TAX, NS-398 and PDTC all reduced SGC7901 growth, with dose- dependence. With increasing dose of TAX, the expression of COX-2, p65 and P-gp showed rising trends, this being reversed by NS-398. PDTC also caused decrease in expression of p65 and P-gp over time. CONCLUSION:COX-2 may induce the expression of P-gp in SGC7901 cell line via the NF-kappa B pathway with pacliaxel stimulation.
Authors: Dong Li; Shu-Hong Hao; Yan Sun; Chun-Mei Hu; Zhi-Hua Ma; Zhi Ming Wang; Jie Liu; Hong Bo Liu; Ming Ye; Yu Fei Zhang; Dong Sheng Yang; Guang Shi Journal: Biomed Res Int Date: 2015-04-21 Impact factor: 3.411
Authors: Anika M S Hartz; Ralf G Rempe; Emma L B Soldner; Anton Pekcec; Juli Schlichtiger; Richard Kryscio; Bjoern Bauer Journal: FASEB J Date: 2019-10-29 Impact factor: 5.834