PURPOSE: Enhanced carnosine levels have been shown to be ergogenic for high-intensity exercise performances, although the role of carnosine in the control of muscle function is poorly understood. Therefore, the aim of this study was to investigate the effect of long-term supplementation with increasing doses of carnosine and beta-alanine on muscle carnosine, anserine, and taurine levels and on in vitro contractility and fatigue in mice. METHODS: Male Naval Medical Research Institute mice (n = 66) were control fed or supplemented with either carnosine (0.1%, 0.5%, or 1.8%) or beta-alanine (0.6 or 1.2%) in their drinking water for 8-12 wk. Soleus and extensor digitorum longus (EDL) were tested for in vitro contractile properties, and carnosine, anserine, and taurine content were measured in EDL and tibialis anterior by high-performance liquid chromatography. RESULTS: Only supplementation with 1.8% carnosine and 1.2% beta-alanine resulted in markedly higher carnosine (up to +160%) and anserine levels (up to +46%) compared with control mice. Beta-alanine supplementation (1.2%) resulted in increased fatigue resistance in the beginning of the fatigue protocol in soleus (+2%-4%) and a marked leftward shift of the force-frequency relation in EDL (10%-31% higher relative forces). CONCLUSION: Comparable with humans, beta-alanine availability seems to be the rate-limiting step for synthesis of muscle histidine-containing dipeptides in mice. Moreover, muscle histidine-containing dipeptides loading in mice moderately and muscle dependently affects excitation-contraction coupling and fatigue.
PURPOSE: Enhanced carnosine levels have been shown to be ergogenic for high-intensity exercise performances, although the role of carnosine in the control of muscle function is poorly understood. Therefore, the aim of this study was to investigate the effect of long-term supplementation with increasing doses of carnosine and beta-alanine on muscle carnosine, anserine, and taurine levels and on in vitro contractility and fatigue in mice. METHODS: Male Naval Medical Research Institute mice (n = 66) were control fed or supplemented with either carnosine (0.1%, 0.5%, or 1.8%) or beta-alanine (0.6 or 1.2%) in their drinking water for 8-12 wk. Soleus and extensor digitorum longus (EDL) were tested for in vitro contractile properties, and carnosine, anserine, and taurine content were measured in EDL and tibialis anterior by high-performance liquid chromatography. RESULTS: Only supplementation with 1.8% carnosine and 1.2% beta-alanine resulted in markedly higher carnosine (up to +160%) and anserine levels (up to +46%) compared with control mice. Beta-alanine supplementation (1.2%) resulted in increased fatigue resistance in the beginning of the fatigue protocol in soleus (+2%-4%) and a marked leftward shift of the force-frequency relation in EDL (10%-31% higher relative forces). CONCLUSION: Comparable with humans, beta-alanine availability seems to be the rate-limiting step for synthesis of muscle histidine-containing dipeptides in mice. Moreover, muscle histidine-containing dipeptides loading in mice moderately and muscle dependently affects excitation-contraction coupling and fatigue.
Authors: Bryan Saunders; Mariana Franchi; Luana Farias de Oliveira; Vinicius da Eira Silva; Rafael Pires da Silva; Vitor de Salles Painelli; Luiz Augusto Riani Costa; Craig Sale; Roger Charles Harris; Hamilton Roschel; Guilherme Giannini Artioli; Bruno Gualano Journal: Eur J Nutr Date: 2018-12-14 Impact factor: 5.614
Authors: Tushar K Bhattacharya; Brandt D Pence; Jessica M Ossyra; Trisha E Gibbons; Samuel Perez; Robert H McCusker; Keith W Kelley; Rodney W Johnson; Jeffrey A Woods; Justin S Rhodes Journal: Physiol Behav Date: 2015-03-19
Authors: Micah Gross; Chris Boesch; Christine S Bolliger; Barbara Norman; Thomas Gustafsson; Hans Hoppeler; Michael Vogt Journal: Eur J Appl Physiol Date: 2013-11-09 Impact factor: 3.078
Authors: Trisha E Gibbons; Brandt D Pence; Geraldine Petr; Jessica M Ossyra; Houston C Mach; Tushar K Bhattacharya; Samuel Perez; Stephen A Martin; Robert H McCusker; Keith W Kelley; Justin S Rhodes; Rodney W Johnson; Jeffrey A Woods Journal: Behav Brain Res Date: 2014-07-05 Impact factor: 3.332