| Literature DB >> 22869741 |
Nubia B Eloy1, Nathalie Gonzalez, Jelle Van Leene, Katrien Maleux, Hannes Vanhaeren, Liesbeth De Milde, Stijn Dhondt, Leen Vercruysse, Erwin Witters, Raphaël Mercier, Laurence Cromer, Gerrit T S Beemster, Han Remaut, Marc C E Van Montagu, Geert De Jaeger, Paulo C G Ferreira, Dirk Inzé.
Abstract
The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of the APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, we describe the identification of a plant-specific negative regulator of the APC/C complex, designated SAMBA. In Arabidopsis thaliana, SAMBA is expressed during embryogenesis and early plant development and plays a key role in organ size control. Samba mutants produced larger seeds, leaves, and roots, which resulted from enlarged root and shoot apical meristems, and, additionally, they had a reduced fertility attributable to a hampered male gametogenesis. Inactivation of SAMBA stabilized A2-type cyclins during early development. Our data suggest that SAMBA regulates cell proliferation during early development by targeting CYCLIN A2 for APC/C-mediated proteolysis.Entities:
Mesh：
Substances：
Year: 2012 PMID： 22869741 PMCID： PMC3427114 DOI： 10.1073/pnas.1211418109
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205