| Literature DB >> 22859440 |
Xander M R van Wijk1, Myrtille J Vallen, Els M van de Westerlo, Arie Oosterhof, Wensi Hao, Elly M Versteeg, Julius Raben, Ronnie G Wismans, Toon F C M Smetsers, Henry B P M Dijkman, Joost Schalkwijk, Toin H van Kuppevelt.
Abstract
Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed this method to structurally characterize GAGs in tissues, including laser-dissected layers of skin and pathological specimens. This method enables the use of the archival paraffin-embedded material for detailed (structural) analysis of GAGs.Entities:
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Year: 2012 PMID： 22859440 DOI： 10.1093/glycob/cws119
Source DB: PubMed Journal: Glycobiology ISSN： 0959-6658 Impact factor: 4.313