| Literature DB >> 28122943 |
Hannes Hinneburg1,2, Petra Korać3, Falko Schirmeister1,2, Slavko Gasparov4,5, Peter H Seeberger1,2, Vlatka Zoldoš3, Daniel Kolarich6.
Abstract
N- and O-glycans are attractive clinical biomarkers as glycosylation changes in response to diseases. The limited availability of defined clinical specimens impedes glyco-biomarker identification and validation in large patient cohorts. Formalin-fixed paraffin-embedded (FFPE) clinical specimens are the common form of sample preservation in clinical pathology, but qualitative and quantitative N- and O-glycomics of such samples has not been feasible to date. Here, we report a highly sensitive and glycan isomer selective method for simultaneous N- and O-glycomics from histopathological slides. As few as 2000 cells isolated from FFPE tissue sections by laser capture microdissection were sufficient for in-depth histopathology-glycomics using porous graphitized carbon nanoLC ESI-MS/MS. N- and O-glycan profiles were similar between unstained and hematoxylin and eosin stained FFPE samples but differed slightly compared with fresh tissue. This method provides the key to unlock glyco-biomarker information from FFPE histopathological tissues archived in pathology laboratories worldwide.Entities:
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Year: 2017 PMID： 28122943 PMCID： PMC5383776 DOI： 10.1074/mcp.M116.062414
Source DB: PubMed Journal: Mol Cell Proteomics ISSN： 1535-9476 Impact factor: 5.911