Literature DB >> 22854718

Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy.

Shizuka Nakayama1, Yiling Luo, Jie Zhou, T Kwaku Dayie, Herman O Sintim.   

Abstract

C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.

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Year:  2012        PMID: 22854718     DOI: 10.1039/c2cc34379g

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN: 1359-7345            Impact factor:   6.222


  23 in total

1.  Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria.

Authors:  Rita L Strack; Wenjiao Song; Samie R Jaffrey
Journal:  Nat Protoc       Date:  2013-12-19       Impact factor: 13.491

Review 2.  Advances in methods for native expression and purification of RNA for structural studies.

Authors:  Robert T Batey
Journal:  Curr Opin Struct Biol       Date:  2014-02-28       Impact factor: 6.809

3.  Split Spinach Aptamer for Highly Selective Recognition of DNA and RNA at Ambient Temperatures.

Authors:  Nanami Kikuchi; Dmitry M Kolpashchikov
Journal:  Chembiochem       Date:  2016-07-15       Impact factor: 3.164

Review 4.  Long-Range Interactions in Riboswitch Control of Gene Expression.

Authors:  Christopher P Jones; Adrian R Ferré-D'Amaré
Journal:  Annu Rev Biophys       Date:  2017-03-30       Impact factor: 12.981

5.  A minimalist biosensor: Quantitation of cyclic di-GMP using the conformational change of a riboswitch aptamer.

Authors:  Colleen A Kellenberger; Jade Sales-Lee; Yuchen Pan; Madalee M Gassaway; Amy E Herr; Ming C Hammond
Journal:  RNA Biol       Date:  2015-06-26       Impact factor: 4.652

6.  Detection of human immunodeficiency virus RNAs in living cells using Spinach RNA aptamers.

Authors:  Brandon D Burch; Carolina Garrido; David M Margolis
Journal:  Virus Res       Date:  2016-11-30       Impact factor: 3.303

Review 7.  Tracking the homeostasis of second messenger cyclic-di-GMP in bacteria.

Authors:  Anushya Petchiappan; Sujay Y Naik; Dipankar Chatterji
Journal:  Biophys Rev       Date:  2020-02-15

8.  RNA-based fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP.

Authors:  Colleen A Kellenberger; Stephen C Wilson; Jade Sales-Lee; Ming C Hammond
Journal:  J Am Chem Soc       Date:  2013-03-21       Impact factor: 15.419

9.  Engineering and characterization of fluorogenic glycine riboswitches.

Authors:  Simon Ketterer; Lukas Gladis; Adnan Kozica; Matthias Meier
Journal:  Nucleic Acids Res       Date:  2016-05-24       Impact factor: 16.971

Review 10.  "Second-generation" fluorogenic RNA-based sensors.

Authors:  Aruni P K K Karunanayake Mudiyanselage; Rigumula Wu; Mark A Leon-Duque; Kewei Ren; Mingxu You
Journal:  Methods       Date:  2019-01-17       Impact factor: 3.608

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