Literature DB >> 22851307

Single-step reconstitution of apo-hemoproteins at the disruption stage of Escherichia coli cells.

Norifumi Kawakami1, Osami Shoji, Yoshihito Watanabe.   

Abstract

Hemoproteins on their metal: We report a novel strategy for the reconstitution of hemoproteins with non-natural metal complexes; simple addition of manganese and ruthenium porphyrin to E. coli cells immediately prior to homogenization yields the reconstituted proteins. We believe that this simple approach could become a standard reconstitution method for hemoproteins.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22851307     DOI： 10.1002/cbic.201200446

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  9 in total

1.  Stereoselective Cyclopropanation of Electron-Deficient Olefins with a Cofactor Redesigned Carbene Transferase Featuring Radical Reactivity.

Authors:  Daniela M Carminati; Rudi Fasan
Journal:  ACS Catal       Date:  2019-09-05       Impact factor: 13.084

2.  Strategies for the expression and characterization of artificial myoglobin-based carbene transferases.

Authors:  Daniela M Carminati; Eric J Moore; Rudi Fasan
Journal:  Methods Enzymol       Date:  2020-08-06       Impact factor: 1.600

3.  Stereoselective olefin cyclopropanation under aerobic conditions with an artificial enzyme incorporating an iron-chlorin e6 cofactor.

Authors:  Gopeekrishnan Sreenilayam; Eric J Moore; Viktoria Steck; Rudi Fasan
Journal:  ACS Catal       Date:  2017-10-09       Impact factor: 13.084

4.  The chlorite dismutase (HemQ) from Staphylococcus aureus has a redox-sensitive heme and is associated with the small colony variant phenotype.

Authors:  Jeffrey A Mayfield; Neal D Hammer; Richard C Kurker; Thomas K Chen; Sunil Ojha; Eric P Skaar; Jennifer L DuBois
Journal:  J Biol Chem       Date:  2013-06-04       Impact factor: 5.157

5.  Metal Substitution Modulates the Reactivity and Extends the Reaction Scope of Myoglobin Carbene Transfer Catalysts.

Authors:  Gopeekrishnan Sreenilayam; Eric J Moore; Viktoria Steck; Rudi Fasan
Journal:  Adv Synth Catal       Date:  2017-04-12       Impact factor: 5.837

6.  Orthogonal Expression of an Artificial Metalloenzyme for Abiotic Catalysis.

Authors:  Evan W Reynolds; Timothy D Schwochert; Matthew W McHenry; John W Watters; Eric M Brustad
Journal:  Chembiochem       Date:  2017-11-09       Impact factor: 3.164

7.  De novo biosynthesis of a nonnatural cobalt porphyrin cofactor in E. coli and incorporation into hemoproteins.

Authors:  Lydia J Perkins; Brian R Weaver; Andrew R Buller; Judith N Burstyn
Journal:  Proc Natl Acad Sci U S A       Date:  2021-04-20       Impact factor: 11.205

8.  Mechanism of Reconstitution/Activation of the Soluble PQQ-Dependent Glucose Dehydrogenase from Acinetobacter calcoaceticus: A Comprehensive Study.

Authors:  Claire Stines-Chaumeil; François Mavré; Brice Kauffmann; Nicolas Mano; Benoît Limoges
Journal:  ACS Omega       Date:  2020-01-23

9.  Abiological catalysis by artificial haem proteins containing noble metals in place of iron.

Authors:  Hanna M Key; Paweł Dydio; Douglas S Clark; John F Hartwig
Journal:  Nature       Date:  2016-06-13       Impact factor: 49.962
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.