Literature DB >> 22842049

Creating an infrastructure for high-throughput high-resolution cryogenic electron microscopy.

Donald C Shrum1, Brent W Woodruff, Scott M Stagg.   

Abstract

New instrumentation for three-dimensional electron microscopy is facilitating an increase in the throughput of data collection and reconstruction. The increase in throughput creates bottlenecks in the workflow for storing and processing the image data. Here we describe the creation and quantify the throughput of a high-throughput infrastructure supporting collection of three-dimensional data collection.
Copyright © 2012 Elsevier Inc. All rights reserved.

Entities:  

Mesh：

Year:  2012        PMID： 22842049      PMCID： PMC3466351          DOI： 10.1016/j.jsb.2012.07.009

Source DB:  PubMed          Journal:  J Struct Biol        ISSN： 1047-8477            Impact factor:   2.867


  22 in total

1.  Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy.

Authors:  Peter B Rosenthal; Richard Henderson
Journal:  J Mol Biol       Date:  2003-10-31       Impact factor: 5.469

2.  Structure of AAV-DJ, a retargeted gene therapy vector: cryo-electron microscopy at 4.5 Å resolution.

Authors:  Thomas F Lerch; Jason K O'Donnell; Nancy L Meyer; Qing Xie; Kenneth A Taylor; Scott M Stagg; Michael S Chapman
Journal:  Structure       Date:  2012-06-21       Impact factor: 5.006

3.  Automated electron microscope tomography using robust prediction of specimen movements.

Authors:  David N Mastronarde
Journal:  J Struct Biol       Date:  2005-10       Impact factor: 2.867

4.  UCSF tomography: an integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction.

Authors:  Shawn Q Zheng; Bettina Keszthelyi; Eric Branlund; John M Lyle; Michael B Braunfeld; John W Sedat; David A Agard
Journal:  J Struct Biol       Date:  2006-06-23       Impact factor: 2.867

5.  3D reconstruction and processing of volumetric data in cryo-electron tomography.

Authors:  Hanspeter Winkler
Journal:  J Struct Biol       Date:  2006-08-11       Impact factor: 2.867

6.  Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV.

Authors:  Christopher R Booth; Joanita Jakana; Wah Chiu
Journal:  J Struct Biol       Date:  2006-09-22       Impact factor: 2.867

7.  Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction.

Authors:  Xing Zhang; Ethan Settembre; Chen Xu; Philip R Dormitzer; Richard Bellamy; Stephen C Harrison; Nikolaus Grigorieff
Journal:  Proc Natl Acad Sci U S A       Date:  2008-01-31       Impact factor: 11.205

Review 8.  Atomic resolution cryo electron microscopy of macromolecular complexes.

Authors:  Z Hong Zhou
Journal:  Adv Protein Chem Struct Biol       Date:  2011       Impact factor: 3.507

Review 9.  The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules.

Authors:  R Henderson
Journal:  Q Rev Biophys       Date:  1995-05       Impact factor: 5.318

10.  Exploration of parameters in cryo-EM leading to an improved density map of the E. coli ribosome.

Authors:  Jamie LeBarron; Robert A Grassucci; Tanvir R Shaikh; William T Baxter; Jayati Sengupta; Joachim Frank
Journal:  J Struct Biol       Date:  2008-06-18       Impact factor: 2.867
View more
  7 in total

1.  The influence of frame alignment with dose compensation on the quality of single particle reconstructions.

Authors:  John M Spear; Alex J Noble; Qing Xie; Duncan R Sousa; Michael S Chapman; Scott M Stagg
Journal:  J Struct Biol       Date:  2015-09-25       Impact factor: 2.867

2.  Structural Heterogeneity in Pre-40S Ribosomes.

Authors:  Matthew C Johnson; Homa Ghalei; Katelyn A Doxtader; Katrin Karbstein; M Elizabeth Stroupe
Journal:  Structure       Date:  2017-01-19       Impact factor: 5.006

3.  Hrr25/CK1δ-directed release of Ltv1 from pre-40S ribosomes is necessary for ribosome assembly and cell growth.

Authors:  Homa Ghalei; Franz X Schaub; Joanne R Doherty; Yoshihiko Noguchi; William R Roush; John L Cleveland; M Elizabeth Stroupe; Katrin Karbstein
Journal:  J Cell Biol       Date:  2015-03-16       Impact factor: 10.539

4.  Sinorhizobium meliloti Phage ΦM9 Defines a New Group of T4 Superfamily Phages with Unusual Genomic Features but a Common T=16 Capsid.

Authors:  Matthew C Johnson; Kelsey B Tatum; Jason S Lynn; Tess E Brewer; Stephen Lu; Brian K Washburn; M Elizabeth Stroupe; Kathryn M Jones
Journal:  J Virol       Date:  2015-08-26       Impact factor: 5.103

5.  Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state.

Authors:  Duncan R Sousa; Scott M Stagg; M Elizabeth Stroupe
Journal:  J Mol Biol       Date:  2013-09-08       Impact factor: 5.469

6.  RELION: implementation of a Bayesian approach to cryo-EM structure determination.

Authors:  Sjors H W Scheres
Journal:  J Struct Biol       Date:  2012-09-19       Impact factor: 2.867

7.  The DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation.

Authors:  Sohail Khoshnevis; Isabel Askenasy; Matthew C Johnson; Maria D Dattolo; Crystal L Young-Erdos; M Elizabeth Stroupe; Katrin Karbstein
Journal:  PLoS Biol       Date:  2016-06-09       Impact factor: 8.029
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.