| Literature DB >> 22826106 |
Yoshitaka Fujihara1, Kazuhiro Kaseda, Naokazu Inoue, Masahito Ikawa, Masaru Okabe.
Abstract
Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.Entities:
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Year: 2012 PMID: 22826106 DOI: 10.1007/s11248-012-9635-x
Source DB: PubMed Journal: Transgenic Res ISSN: 0962-8819 Impact factor: 2.788