Literature DB >> 10338148

Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein.

T Nakanishi1, M Ikawa, S Yamada, M Parvinen, T Baba, Y Nishimune, M Okabe.   

Abstract

We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ability and the integrity of their acrosome was easily examined in a non-invasive manner by tracing the GFP in individual 'live' sperm with fluorescent microscopy. The time required for the dispersal of acrosomal contents was demonstrated to be approximately 3 s after the onset of acrosome reaction.

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Year:  1999        PMID： 10338148     DOI： 10.1016/s0014-5793(99)00433-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  63 in total

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2.  STAP cells are derived from ES cells.

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Journal:  Nature       Date:  2015-09-24       Impact factor: 49.962

3.  The "soluble" adenylyl cyclase in sperm mediates multiple signaling events required for fertilization.

Authors:  Kenneth C Hess; Brian H Jones; Becky Marquez; Yanqiu Chen; Teri S Ord; Margarita Kamenetsky; Catarina Miyamoto; Jonathan H Zippin; Gregory S Kopf; Susan S Suarez; Lonny R Levin; Carmen J Williams; Jochen Buck; Stuart B Moss
Journal:  Dev Cell       Date:  2005-08       Impact factor: 12.270

4.  Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia.

Authors:  Kazutoshi Fujita; Hiroshi Ohta; Akira Tsujimura; Tetsuya Takao; Yasushi Miyagawa; Shingo Takada; Kiyomi Matsumiya; Teruhiko Wakayama; Akihiko Okuyama
Journal:  J Clin Invest       Date:  2005-06-16       Impact factor: 14.808

5.  Recombinant mouse sperm ZP3-binding protein (ZP3R/sp56) forms a high order oligomer that binds eggs and inhibits mouse fertilization in vitro.

Authors:  Mariano G Buffone; Tiangang Zhuang; Teri S Ord; Ling Hui; Stuart B Moss; George L Gerton
Journal:  J Biol Chem       Date:  2008-03-03       Impact factor: 5.157

6.  Extracellular adenosine 5'-triphosphate alters motility and improves the fertilizing capability of mouse sperm.

Authors:  Esmeralda Rodríguez-Miranda; Mariano G Buffone; Scott E Edwards; Teri S Ord; Kathleen Lin; Mary D Sammel; George L Gerton; Stuart B Moss; Carmen J Williams
Journal:  Biol Reprod       Date:  2008-04-09       Impact factor: 4.285

7.  In vitro sperm production from mouse spermatogonial stem cell lines using an organ culture method.

Authors:  Takuya Sato; Kumiko Katagiri; Yoshinobu Kubota; Takehiko Ogawa
Journal:  Nat Protoc       Date:  2013-10-03       Impact factor: 13.491

8.  Monitoring dimeric status of IZUMO1 during the acrosome reaction in living spermatozoon.

Authors:  Naokazu Inoue; Ikuo Wada
Journal:  Cell Cycle       Date:  2018-07-25       Impact factor: 4.534

9.  Disruption of mouse CD46 causes an accelerated spontaneous acrosome reaction in sperm.

Authors:  Naokazu Inoue; Masahito Ikawa; Tomoko Nakanishi; Misako Matsumoto; Midori Nomura; Tsukasa Seya; Masaru Okabe
Journal:  Mol Cell Biol       Date:  2003-04       Impact factor: 4.272

10.  Viral transduction of male germline stem cells results in transgene transmission after germ cell transplantation in pigs.

Authors:  Wenxian Zeng; Lin Tang; Alla Bondareva; Ali Honaramooz; Valeria Tanco; Camila Dores; Susan Megee; Mark Modelski; Jose Rafael Rodriguez-Sosa; Melissa Paczkowski; Elena Silva; Matt Wheeler; Rebecca L Krisher; Ina Dobrinski
Journal:  Biol Reprod       Date:  2013-01-31       Impact factor: 4.285
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